Human Interferon Lambda-1, IFNL1 ELISA Kit

Interleukin-29 (IL-29), also known as cytokine Zcyto21, Interferon lambda-1, IFN-lambda-1, and IFNL1, is a secreted protein that belongs to the IL-28 / IL-29 family. IL-29 is a cytokine with immunomodulatory activity. IL-29 is highly similar in amino acid sequence to the IL-28. IL-28 and IL-29 are induced by a viral infection and showed antiviral activity. IL-28 and IL-29 interacted with a heterodimeric class II cytokine receptor that consisted of IL-10 receptor beta (IL-10Rbeta) and an orphan class II receptor chain, designated IL-28Ralpha. IL-29 plays an important role in host defenses against microbes and its gene is highly upregulated in cells infected with viruses. IL-29 may play a role in antiviral immunity. IL-29 up-regulates MHC class I antigen expression. It is a Ligand for the heterodimeric class II cytokine receptor composed of IL10RB and IL28RA. The ligand/receptor complex seems to signal through the Jak-STAT pathway.

Concentration | O.D. | Average | Corrected ------------------------------------------ 450 pg/ml | 2.382 | 2.437 | 2.347 2.492 225 pg/ml | 1.432 | 1.389 | 1.299 1.346 112.5 pg/ml | 0.822 | 0.783 | 0.693 0.743 56.25 pg/ml | 0.446 | 0.462 | 0.372 0.477 28.125 pg/ml | 0.277 | 0.278 | 0.188 0.279 14.0625 pg/ml | 0.174 | 0.184 | 0.094 0.193 7.03125 pg/ml | 0.14 | 0.14 | 0.05 0.139 0 pg/ml | 0.086 | 0.09 | 0 0.094

Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% ---------------------------------------------------------------- Intra-assay | 1 | 20 | 27.7 | 0.76 | 2.7 Intra-assay | 2 | 20 | 65.9 | 2.2 | 3.3 Intra-assay | 3 | 20 | 244 | 8.1 | 3.3 Inter-assay | 1 | 20 | 27.5 | 2.3 | 8.4 Inter-assay | 2 | 20 | 65.5 | 4.4 | 6.7 Inter-assay | 3 | 20 | 247 | 10.3 | 4.2

Components | Quantity --------------------- Pre-coated ELISA Plate | 12 x 8 well strips x 1 Standard Solution | 0.2mL x 1 vail Dilution Buffer | 20mL x 1 vail Stop Solution | 6mL x 1 vail Substrate Solution A | 12mL x 1 vail Substrate Solution B | 12mL x 1 vail Wash Buffer Concentrate (25x) | 20mL x 1 vail Detection Antibody Concentrate | 0.6mL x 1 vail