Human Basigin, BSG ELISA Kit

CD147/EMMPRIN (Extracellular Matrix Metalloproteinase Inducer), also known as Basigin (BSG), is a transmembrane glycoprotein with different forms resulted from different modes of glycosylation and N-terminal sequence variants. It is a member of the immunoglobulin superfamily with homology to both the immunoglobulin V domain and MHC class II antigen beta-chain. This protein play important roles in variety of events including spermatogenesis, embryo implantation, neural network formation. CD147 induces the production and release of matrix metalloproteinases (MMP) in the surrounding mesenchymal cells and tumor cells, and thereby promotes invasion, metastasis, growth and survival of malignant cells. Furthermore, CD147 also serves as a receptor for extracellular cyclophilinthe and its association with integrins might be important in signal transduction. Recently, CD147 displays increased expression in many cancers, and it has been previously demonstrated to participate in cancer metastasis and progression. Thus, CD147 and its antibody are used as an effective treatment for malignant cancers.

Concentration | O.D. | Average | Corrected ------------------------------------------ 400 pg/ml | 1.792 | 1.795 | 1.689 1.798 200 pg/ml | 1.284 | 1.252 | 1.146 1.219 100 pg/ml | 0.839 | 0.838 | 0.732 0.837 50 pg/ml | 0.515 | 0.523 | 0.417 0.531 25 pg/ml | 0.326 | 0.33 | 0.224 0.334 12.5 pg/ml | 0.212 | 0.221 | 0.115 0.23 6.25 pg/ml | 0.16 | 0.166 | 0.06 0.171 0 pg/ml | 0.105 | 0.106 | 0 0.106

Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% ---------------------------------------------------------------- Intra-assay | 1 | 20 | 21.5 | 1.2 | 5.6 Intra-assay | 2 | 20 | 63.2 | 1.7 | 2.7 Intra-assay | 3 | 20 | 218 | 5.2 | 2.4 Inter-assay | 1 | 20 | 21.5 | 2.2 | 10.1 Inter-assay | 2 | 20 | 62.6 | 6.2 | 10 Inter-assay | 3 | 20 | 216 | 20 | 9.2

Components | Quantity --------------------- Pre-coated ELISA Plate | 12 x 8 well strips x 1 Standard Solution | 0.2mL x 1 vail Dilution Buffer | 20mL x 1 vail Stop Solution | 6mL x 1 vail Substrate Solution A | 12mL x 1 vail Substrate Solution B | 12mL x 1 vail Wash Buffer Concentrate (25x) | 20mL x 1 vail Detection Antibody Concentrate | 0.6mL x 1 vail