Human Carboxypeptidase B, CPB1 ELISA Kit

Carboxypeptidase B1, also well known as pancreatic procarboxypeptidase B (PCPB), is a highly pancreas -specific protein (PASP), and has been identified previously as a serum marker for acute pancreatitis and pancreatic graft rejection. As the prototype for those human exopeptidases that cleave off basic C-terminal residues, CPB1 specifically cleaves the C-terminal Arg and Lys residues with a preference for Arg. The B1 and B2 forms of procarboxypeptidase B differ from each other mainly in isoelectric point.The deduced amino acid sequence of PCPB predicts a 416-amino acid preproenzyme consisting of a 15-aa signal peptide, a 95-aa activation peptide and a 37-aa mature chain. The secreted PCPB zymogen is converted to enzymatically active CPB1 by limited proteolysis by trypsin.

Concentration | O.D. | Average | Corrected ------------------------------------------ 400 pg/ml | 2.877 | 2.971 | 2.872 3.064 200 pg/ml | 1.526 | 1.463 | 1.364 1.399 100 pg/ml | 0.836 | 0.783 | 0.684 0.73 50 pg/ml | 0.408 | 0.433 | 0.334 0.457 25 pg/ml | 0.273 | 0.274 | 0.175 0.275 12.5 pg/ml | 0.171 | 0.184 | 0.085 0.196 6.25 pg/ml | 0.142 | 0.135 | 0.036 0.127 0 pg/ml | 0.097 | 0.099 | 0 0.1

Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% ---------------------------------------------------------------- Intra-assay | 1 | 20 | 17 | 1.2 | 6.8 Intra-assay | 2 | 20 | 70.8 | 3.2 | 4.5 Intra-assay | 3 | 20 | 208 | 7.9 | 3.8 Inter-assay | 1 | 20 | 17.1 | 1.5 | 8.9 Inter-assay | 2 | 20 | 71.4 | 3.8 | 5.3 Inter-assay | 3 | 20 | 209 | 14.8 | 7.1

Components | Quantity --------------------- Pre-coated ELISA Plate | 12 x 8 well strips x 1 Standard Solution | 0.2mL x 1 vail Dilution Buffer | 20mL x 1 vail Stop Solution | 6mL x 1 vail Substrate Solution A | 12mL x 1 vail Substrate Solution B | 12mL x 1 vail Wash Buffer Concentrate (25x) | 20mL x 1 vail Detection Antibody Concentrate | 0.6mL x 1 vail