Human C-C Motif Chemokine 23, CCL23 ELISA Kit

CCL23, also known as MIP 3, is a small cytokine which belongs to the CC chemokine family. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. CCL23 is predominantly expressed in lung and liver tissue, but also can be detected in bone marrow and placenta. It displays chemotactic activity on resting T lymphocytes and monocytes, lower activity on neutrophils and no activity on activated T lymphocytes. CCL23 is also a strong suppressor of colony formation by a multipotential hematopoietic progenitor cell line.

Concentration | O.D. | Average | Corrected ------------------------------------------ 400 pg/ml | 2.562 | 2.513 | 2.42 2.465 200 pg/ml | 1.404 | 1.473 | 1.38 1.542 100 pg/ml | 0.797 | 0.832 | 0.739 0.867 50 pg/ml | 0.458 | 0.464 | 0.371 0.47 25 pg/ml | 0.244 | 0.252 | 0.159 0.261 12.5 pg/ml | 0.19 | 0.181 | 0.088 0.173 6.25 pg/ml | 0.132 | 0.137 | 0.044 0.142 0 pg/ml | 0.094 | 0.093 | 0 0.092

Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% ---------------------------------------------------------------- Intra-assay | 1 | 20 | 59.3 | 3.4 | 5.7 Intra-assay | 2 | 20 | 121 | 4.3 | 3.5 Intra-assay | 3 | 20 | 275 | 14.5 | 5.3 Inter-assay | 1 | 20 | 59.1 | 3.7 | 6.3 Inter-assay | 2 | 20 | 121 | 12.4 | 10.3 Inter-assay | 3 | 20 | 274 | 8.9 | 3.3

Components | Quantity --------------------- Pre-coated ELISA Plate | 12 x 8 well strips x 1 Standard Solution | 0.2mL x 1 vail Dilution Buffer | 20mL x 1 vail Stop Solution | 6mL x 1 vail Substrate Solution A | 12mL x 1 vail Substrate Solution B | 12mL x 1 vail Wash Buffer Concentrate (25x) | 20mL x 1 vail Detection Antibody Concentrate | 0.6mL x 1 vail