Human Interleukin-20, IL20 ELISA Kit

IL20/Interleukin-20 belongs to the IL-10 family. It is a cytokine structurally related to interleukin 10. IL20/Interleukin-20 can be detected in skin, trachea, and other tissues. It is produced by activated keratinocytes and monocytes and transmits an intracellular signal through two distinct cell-surface receptor complexes on keratinocytes and other epithelial cells. It has been shown that interleukin-20 transduces its signal through signal transducer and activator of transcription 3 (STAT3) in keratinocytes. It may be involved in epidermal function and psoriasis. It also regulates the proliferation and differentiation of keratinocytes during inflammation, particularly inflammation associated with the skin. Also, IL20/Interleukin-20 also causes cell expansion of multipotential hematopoietic progenitor cells. A specific receptor for this cytokine is found to be expressed in skin and upregulated dramatically in psoriatic skin, suggesting a role for this protein in epidermal function and psoriasis.

Concentration | O.D. | Average | Corrected ------------------------------------------ 400 pg/ml | 2.546 | 2.431 | 2.328 2.316 200 pg/ml | 1.284 | 1.337 | 1.234 1.389 100 pg/ml | 0.756 | 0.708 | 0.605 0.659 50 pg/ml | 0.382 | 0.386 | 0.283 0.39 25 pg/ml | 0.217 | 0.231 | 0.128 0.244 12.5 pg/ml | 0.167 | 0.174 | 0.071 0.18 6.25 pg/ml | 0.131 | 0.137 | 0.034 0.143 0 pg/ml | 0.106 | 0.103 | 0 0.1

Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% ---------------------------------------------------------------- Intra-assay | 1 | 20 | 27.6 | 0.73 | 2.7 Intra-assay | 2 | 20 | 120 | 3.3 | 2.8 Intra-assay | 3 | 20 | 292 | 9.1 | 3.1 Inter-assay | 1 | 20 | 27.4 | 1.9 | 6.8 Inter-assay | 2 | 20 | 121 | 7.1 | 5.9 Inter-assay | 3 | 20 | 291 | 23.1 | 7.9

Components | Quantity --------------------- Pre-coated ELISA Plate | 12 x 8 well strips x 1 Standard Solution | 0.2mL x 1 vail Dilution Buffer | 20mL x 1 vail Stop Solution | 6mL x 1 vail Substrate Solution A | 12mL x 1 vail Substrate Solution B | 12mL x 1 vail Wash Buffer Concentrate (25x) | 20mL x 1 vail Detection Antibody Concentrate | 0.6mL x 1 vail