Mouse Cd5 Antigen-Like, Cd5l ELISA Kit

CD5L, also known as CD5 antigen-like, is a soluble protein belonging to group B of the scavenger receptor cysteine-rich (SRCR) superfamily and contains three SRCR domains. It is a secreted glycoprotein and expressed by macrophages presentin lymphoid tissues (spleen, lymph node, thymus, and bone marrow) . It binds to myelomonocytic and lymphoid cells and may play an important role in the regulation of the innate and adaptive immune systems. CD5L functions as a pattern recognition molecule by binding both lipoteichoic acid (LTA) on Gram positive and lipopolysaccharide (LPS) on Gram negative bacteria. and the SRCR domain 1 of CD5L retains both the LPS and LTA binding activities. In addtion, it is revealed that CD5L seems to play a role as an inhibitor of apoptosis.

Concentration | O.D. | Average | Corrected ------------------------------------------ 400 pg/ml | 1.464 | 1.525 | 1.415 1.586 200 pg/ml | 0.987 | 0.923 | 0.813 0.859 100 pg/ml | 0.511 | 0.535 | 0.425 0.559 50 pg/ml | 0.305 | 0.325 | 0.215 0.345 25 pg/ml | 0.209 | 0.197 | 0.087 0.185 12.5 pg/ml | 0.144 | 0.153 | 0.043 0.162 6.25 pg/ml | 0.139 | 0.133 | 0.023 0.127 0 pg/ml | 0.11 | 0.11 | 0 0.109

Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% ---------------------------------------------------------------- Intra-assay | 1 | 20 | 29.6 | 1.5 | 5.1 Intra-assay | 2 | 20 | 82.1 | 2.8 | 3.4 Intra-assay | 3 | 20 | 293 | 7.5 | 2.5 Inter-assay | 1 | 20 | 29.7 | 2.6 | 8.8 Inter-assay | 2 | 20 | 81.7 | 8.3 | 10.2 Inter-assay | 3 | 20 | 290 | 26.9 | 9.3

Components | Quantity --------------------- Pre-coated ELISA Plate | 12 x 8 well strips x 1 Standard Solution | 0.2mL x 1 vail Dilution Buffer | 20mL x 1 vail Stop Solution | 6mL x 1 vail Substrate Solution A | 12mL x 1 vail Substrate Solution B | 12mL x 1 vail Wash Buffer Concentrate (25x) | 20mL x 1 vail Detection Antibody Concentrate | 0.6mL x 1 vail