Mouse Interleukin-1 Alpha, Il1a ELISA Kit

IL-1 alpha is a member of the interleukin 1 cytokine family. Cytokines are proteinaceous signaling compounds that are major mediators of the immune response. They control many different cellular functions including proliferation, differentiation, and cell survival/apoptosis but are also involved in several pathophysiological processes including viral infections and autoimmune diseases. Cytokines are synthesized under various stimuli by a variety of cells of both the innate (monocytes, macrophages, dendritic cells) and adaptive (T- and B-cells) immune systems. Cytokines can be classified into two groups: pro- and anti-inflammatory. Pro-inflammatory cytokines, including IFNgamma, IL-1, IL-6, and TNF-alpha, are predominantly derived from the innate immune cells and Th1 cells. Anti-inflammatory cytokines, including IL-10, IL-4, IL-13, and IL-5, are synthesized from Th2 immune cells. IL-1 alpha is a pleiotropic cytokine involved in various immune responses, inflammatory processes, and hematopoiesis. It is produced by monocytes and macrophages as a proprotein, which is proteolytically processed and released in response to cell injury, and thus induces apoptosis. IL-1 alpha stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. Cancer Immunotherapy Immune Checkpoint Immunotherapy Targeted Therapy

Concentration | O.D. | Average | Corrected ------------------------------------------ 400 pg/ml | 2.842 | 2.811 | 2.677 2.78 200 pg/ml | 1.628 | 1.579 | 1.445 1.531 100 pg/ml | 0.911 | 0.86 | 0.726 0.81 50 pg/ml | 0.506 | 0.499 | 0.365 0.492 25 pg/ml | 0.304 | 0.313 | 0.179 0.322 12.5 pg/ml | 0.23 | 0.222 | 0.088 0.214 6.25 pg/ml | 0.17 | 0.177 | 0.043 0.184 0 pg/ml | 0.142 | 0.134 | 0 0.126

Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% ---------------------------------------------------------------- Intra-assay | 1 | 20 | 25.6 | 1.8 | 7.1 Intra-assay | 2 | 20 | 98.3 | 4.1 | 4.2 Intra-assay | 3 | 20 | 266 | 10.1 | 3.8 Inter-assay | 1 | 20 | 25.8 | 1.6 | 6.4 Inter-assay | 2 | 20 | 99.5 | 9.3 | 9.3 Inter-assay | 3 | 20 | 265 | 21.3 | 8

Components | Quantity --------------------- Pre-coated ELISA Plate | 12 x 8 well strips x 1 Standard Solution | 0.2mL x 1 vail Dilution Buffer | 20mL x 1 vail Stop Solution | 6mL x 1 vail Substrate Solution A | 12mL x 1 vail Substrate Solution B | 12mL x 1 vail Wash Buffer Concentrate (25x) | 20mL x 1 vail Detection Antibody Concentrate | 0.6mL x 1 vail