Mouse Interleukin-6 Receptor Subunit Alpha, Il6ra ELISA Kit

Interleukin 6 receptor (IL-6R) also known as CD126 (Cluster of Differentiation 126) is a type I cytokine receptor. The low concentration of a soluble form of IL-6 receptor (SIL-6R) acts as an agonist of IL-6 activity. In the IL-6R/CD126/IL6R system, both a membrane-bound IL-6R and a SIL-6R protein can mediate IL-6 signals into the cells through the interaction of gp130. The resulting IL-6/sIL-6R protein complex is also capable of binding to gp130 and inducing intracellular signaling. Through this so-called 'trans-signaling' mechanism, IL-6 can stimulate cells that lack an endogenous mIL-6R. High levels of IL-6 and SIL-6R have been reported in several chronic inflammatory and autoimmune diseases as well as in cancer. Cancer Immunotherapy Immune Checkpoint Immunotherapy Targeted Therapy

Concentration | O.D. | Average | Corrected ------------------------------------------ 400 pg/ml | 2.833 | 2.737 | 2.618 2.641 200 pg/ml | 1.535 | 1.577 | 1.458 1.619 100 pg/ml | 0.753 | 0.758 | 0.639 0.763 50 pg/ml | 0.445 | 0.417 | 0.298 0.39 25 pg/ml | 0.303 | 0.284 | 0.165 0.266 12.5 pg/ml | 0.207 | 0.2 | 0.081 0.194 6.25 pg/ml | 0.169 | 0.159 | 0.04 0.149 0 pg/ml | 0.114 | 0.119 | 0 0.125

Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% ---------------------------------------------------------------- Intra-assay | 1 | 20 | 18 | 1.1 | 5.9 Intra-assay | 2 | 20 | 48.7 | 2.8 | 5.8 Intra-assay | 3 | 20 | 222 | 15.5 | 7 Inter-assay | 1 | 20 | 18.1 | 1.4 | 7.8 Inter-assay | 2 | 20 | 48.5 | 4.8 | 10 Inter-assay | 3 | 20 | 225 | 14.6 | 6.5

Components | Quantity --------------------- Pre-coated ELISA Plate | 12 x 8 well strips x 1 Standard Solution | 0.2mL x 1 vail Dilution Buffer | 20mL x 1 vail Stop Solution | 6mL x 1 vail Substrate Solution A | 12mL x 1 vail Substrate Solution B | 12mL x 1 vail Wash Buffer Concentrate (25x) | 20mL x 1 vail Detection Antibody Concentrate | 0.6mL x 1 vail