Mouse Cd209 Antigen-Like Protein B, Cd209b ELISA Kit

The cluster of differentiation (CD) system is commonly used as cell markers in Immunophenotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules associating with the immune function of the cell. There are more than 320 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. CD209b, also known as SIGNR1, is a C-type lectin receptor. CD209b is present on most regions of mouse brain and found on microglia and dendritic cells but not on neurons or astrocytes. CD209b is implicated in the recently described SIGNR1 complement activation pathway, which operates against capsular polysaccharides in splenic marginal macrophages. CD209b in rat is homologue to SIGNR1 in mouse, both of which are found to mediate the uptake of dextran or CPS14 within the splenic marginal zone.

Concentration | O.D. | Average | Corrected ------------------------------------------ 400 pg/ml | 3.133 | 3.08 | 2.96 3.027 200 pg/ml | 1.642 | 1.682 | 1.562 1.722 100 pg/ml | 0.828 | 0.881 | 0.761 0.934 50 pg/ml | 0.525 | 0.497 | 0.377 0.469 25 pg/ml | 0.309 | 0.307 | 0.187 0.305 12.5 pg/ml | 0.219 | 0.213 | 0.093 0.207 6.25 pg/ml | 0.154 | 0.16 | 0.04 0.166 0 pg/ml | 0.114 | 0.12 | 0 0.126

Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% ---------------------------------------------------------------- Intra-assay | 1 | 20 | 47.2 | 2.8 | 6 Intra-assay | 2 | 20 | 98.3 | 4.4 | 4.5 Intra-assay | 3 | 20 | 289 | 12.9 | 4.5 Inter-assay | 1 | 20 | 46.7 | 4.6 | 10 Inter-assay | 2 | 20 | 99.1 | 7.1 | 7.2 Inter-assay | 3 | 20 | 287 | 17.5 | 6.1

Components | Quantity --------------------- Pre-coated ELISA Plate | 12 x 8 well strips x 1 Standard Solution | 0.2mL x 1 vail Dilution Buffer | 20mL x 1 vail Stop Solution | 6mL x 1 vail Substrate Solution A | 12mL x 1 vail Substrate Solution B | 12mL x 1 vail Wash Buffer Concentrate (25x) | 20mL x 1 vail Detection Antibody Concentrate | 0.6mL x 1 vail