Human Tumor-Associated Calcium Signal Transducer 2, TACSTD2 ELISA Kit

TROP-2, also referred to as tumor-associated calcium signal transducer 2 (TACSTD2), GA733-1 or M1S1, is a cell surface glycoprotein highly expressed in a wide variety of epithelial cancers. In contrast, there is little or no expression of Trop-2 in adult somatic tissue. Because it is a cell surface protein that is selectively expressed in tumor cells, Trop-2 is a potential therapeutic target. The cytoplasmic tail of Trop-2 possesses potential serine and tyrosine phosphorylation sites and a phosphatidyl-inositol binding consensus sequence. Trop-2 transduces an intracellular calcium signal, which are consistent with the hypothesis that it acts as a cell surface receptor and support a search for a physiological ligand. TROP2 encoding by an intronless gene was originally defined by the monoclonal antibody GA733, and is a member of a family of at least two type I membrane proteins. The other known member is GA733-2, also called EpCAM and TROP1. It has been suggested by studies that the GA733-1 gene was formed by the retroposition of the GA733-2 gene via an mRNA intermediate.

Concentration | O.D. | Average | Corrected ------------------------------------------ 350 pg/ml | 2.506 | 2.587 | 2.486 2.669 175 pg/ml | 1.352 | 1.409 | 1.308 1.467 87.5 pg/ml | 0.83 | 0.784 | 0.683 0.739 43.75 pg/ml | 0.457 | 0.445 | 0.344 0.434 21.875 pg/ml | 0.283 | 0.278 | 0.177 0.274 10.9375 pg/ml | 0.18 | 0.189 | 0.088 0.199 5.46875 pg/ml | 0.135 | 0.144 | 0.043 0.154 0 pg/ml | 0.095 | 0.101 | 0 0.108

Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% ---------------------------------------------------------------- Intra-assay | 1 | 20 | 45.8 | 3 | 6.5 Intra-assay | 2 | 20 | 124 | 5.8 | 4.6 Intra-assay | 3 | 20 | 252 | 7.5 | 3 Inter-assay | 1 | 20 | 45.7 | 3.3 | 7.3 Inter-assay | 2 | 20 | 125 | 6.4 | 5.1 Inter-assay | 3 | 20 | 251 | 11.2 | 4.5

Components | Quantity --------------------- Pre-coated ELISA Plate | 12 x 8 well strips x 1 Standard Solution | 0.2mL x 1 vail Dilution Buffer | 20mL x 1 vail Stop Solution | 6mL x 1 vail Substrate Solution A | 12mL x 1 vail Substrate Solution B | 12mL x 1 vail Wash Buffer Concentrate (25x) | 20mL x 1 vail Detection Antibody Concentrate | 0.6mL x 1 vail