Human Leukocyte-Associated Immunoglobulin-Like Receptor 2, LAIR2 ELISA Kit

Leukocyte-associated immunoglobulin-like receptor 2 (LAIR2), also known as CD36, is a 131 amino acid protein containing one Ig-like C2-type domain. It is expressed as a soluble receptor exhibiting high affinity for various collagen molecules to which it binds in a hydroxyproline-dependent manner. LAIR2 is a member of the immunoglobulin superfamily and was identified by its similarity to LAIR1, an inhibitory receptor present on mononuclear leukocytes. LAIR2 is thought to be secreted and may help modulate mucosal tolerance. As a natural competitor for LAIR1, soluble LAIR2 prevents binding of human LAIR1 to collagens and LAIR1 cross-linking, thereby regulating its inhibitory potential. Accordingly, LAIR2 is suggested to perform an immunoregulatory function.

Concentration | O.D. | Average | Corrected ------------------------------------------ 350 pg/ml | 2.418 | 2.343 | 2.229 2.268 175 pg/ml | 1.339 | 1.351 | 1.237 1.363 87.5 pg/ml | 0.738 | 0.72 | 0.606 0.702 43.75 pg/ml | 0.423 | 0.421 | 0.307 0.419 21.875 pg/ml | 0.287 | 0.275 | 0.161 0.263 10.9375 pg/ml | 0.209 | 0.205 | 0.091 0.201 5.46875 pg/ml | 0.136 | 0.146 | 0.032 0.156 0 pg/ml | 0.111 | 0.114 | 0 0.117

Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% ---------------------------------------------------------------- Intra-assay | 1 | 20 | 39.5 | 1.7 | 4.4 Intra-assay | 2 | 20 | 110 | 3.5 | 3.2 Intra-assay | 3 | 20 | 224 | 6.7 | 3 Inter-assay | 1 | 20 | 39.6 | 3.1 | 7.8 Inter-assay | 2 | 20 | 110 | 8.5 | 7.7 Inter-assay | 3 | 20 | 226 | 12.4 | 5.5

Components | Quantity --------------------- Pre-coated ELISA Plate | 12 x 8 well strips x 1 Standard Solution | 0.2mL x 1 vail Dilution Buffer | 20mL x 1 vail Stop Solution | 6mL x 1 vail Substrate Solution A | 12mL x 1 vail Substrate Solution B | 12mL x 1 vail Wash Buffer Concentrate (25x) | 20mL x 1 vail Detection Antibody Concentrate | 0.6mL x 1 vail