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Human Interleukin-7, IL7 ELISA Kit

Product Specifications

Background

IL7, also known as interleukin 7, is a hematopoietic growth factor that belongs to the IL-7/IL-9 family. It is secreted by stromal cells in the bone marrow and thymus. IL7 stimulates the proliferation of lymphoid progenitors. It is important for proliferation during certain stages of B-cell maturation. IL7 and the hepatocyte growth factor (HGF) form a heterodimer that functions as a pre-pro-B cell growth-stimulating factor. It is found to be a cofactor for V (D) J rearrangement of the T cell receptor beta (TCRß) during early T cell development. IL7 can be produced locally by intestinal epithelial and epithelial goblet cells and may serve as a regulatory factor for intestinal mucosal lymphocytes. Cancer Immunotherapy Immune Checkpoint Immunotherapy Targeted Therapy

Product Name Alternative

Interleukin-7

Synonyms

LP-1

Gene Name

IL7

Gene ID

3574

UniProt

P13232

Target

IL-7

Field of Research

Immunology, Neuroscience, Cancer

Assay Type

Sandwich

Assay Protocol

Concentration | O.D. | Average | Corrected ------------------------------------------ 350 pg/ml | 2.512 | 2.608 | 2.49 2.704 175 pg/ml | 1.383 | 1.421 | 1.303 1.459 87.5 pg/ml | 0.867 | 0.818 | 0.7 0.769 43.75 pg/ml | 0.498 | 0.479 | 0.361 0.46 21.875 pg/ml | 0.305 | 0.294 | 0.176 0.283 10.9375 pg/ml | 0.209 | 0.2 | 0.082 0.19 5.46875 pg/ml | 0.158 | 0.155 | 0.037 0.152 0 pg/ml | 0.124 | 0.118 | 0 0.112

Assay Performance Time

3.5h

Sample Type

Serum, plasma, cell culture supernates

Detection Range

5.47-350 pg/mL

Precision

Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% ---------------------------------------------------------------- Intra-assay | 1 | 20 | 18.8 | 1 | 5.4 Intra-assay | 2 | 20 | 58.7 | 1.5 | 2.5 Intra-assay | 3 | 20 | 279 | 6.6 | 2.4 Inter-assay | 1 | 20 | 18.8 | 1.9 | 10.2 Inter-assay | 2 | 20 | 59.2 | 5.9 | 10 Inter-assay | 3 | 20 | 282 | 25.9 | 9.2

Sensitivity

2.43 pg/mL

Components

Components | Quantity --------------------- Pre-coated ELISA Plate | 12 x 8 well strips x 1 Standard Solution | 0.2mL x 1 vail Dilution Buffer | 20mL x 1 vail Stop Solution | 6mL x 1 vail Substrate Solution A | 12mL x 1 vail Substrate Solution B | 12mL x 1 vail Wash Buffer Concentrate (25x) | 20mL x 1 vail Detection Antibody Concentrate | 0.6mL x 1 vail

Storage Temperature

2-8°C

Species

Human

Mass

20187

Available Sizes

Frequently Asked Questions

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