Human Interleukin-13, IL13 ELISA Kit

Interleukin 13 (IL-13) is a single-chain glycosylated polypeptide, which belongs to the IL-13/IL-4 family. IL-13 protein is secreted by many cell types, but especially by T helper type 2 (Th2) cells. IL-13 exerts its effects through a multi-subunit receptor comprising the alpha chain of the IL-4 receptor (IL-4Rα) and at least one of two known IL-13-specific binding chains (IL-13 Rα1 and IL-13 Rα2) . As a cytokine, IL-13 protein is critical in regulating inflammatory, immune responses, and diseases. Also, it inhibits the production of pro-inflammatory cytokines and chemokines, and thus down-regulates macrophage activity. IL-13 protein and antibody are more importantly implicated as a central mediator of immunoregulatory processes in various cell types. Cancer Immunotherapy Immune Checkpoint Immunotherapy Targeted Therapy

Concentration | O.D. | Average | Corrected ------------------------------------------ 300 pg/ml | 2.599 | 2.558 | 2.437 2.516 150 pg/ml | 1.412 | 1.399 | 1.278 1.386 75 pg/ml | 0.763 | 0.791 | 0.67 0.818 37.5 pg/ml | 0.406 | 0.435 | 0.314 0.464 18.75 pg/ml | 0.286 | 0.274 | 0.153 0.261 9.375 pg/ml | 0.199 | 0.197 | 0.076 0.194 4.6875 pg/ml | 0.151 | 0.155 | 0.034 0.159 0 pg/ml | 0.124 | 0.121 | 0 0.117

Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% ---------------------------------------------------------------- Intra-assay | 1 | 20 | 25 | 2.3 | 9.2 Intra-assay | 2 | 20 | 51.1 | 4.7 | 9.3 Intra-assay | 3 | 20 | 222 | 10.7 | 4.8 Inter-assay | 1 | 20 | 25 | 2.4 | 9.6 Inter-assay | 2 | 20 | 51.2 | 4.4 | 8.6 Inter-assay | 3 | 20 | 224 | 16.4 | 7.3

Components | Quantity --------------------- Pre-coated ELISA Plate | 12 x 8 well strips x 1 Standard Solution | 0.2mL x 1 vail Dilution Buffer | 20mL x 1 vail Stop Solution | 6mL x 1 vail Substrate Solution A | 12mL x 1 vail Substrate Solution B | 12mL x 1 vail Wash Buffer Concentrate (25x) | 20mL x 1 vail Detection Antibody Concentrate | 0.6mL x 1 vail