Rat T-Lymphocyte Activation Antigen Cd86 ELISA Kit

CD86, also known as B-lymphocyte activation antigen B7-2 (referred to as B70), is a member of the cell surface immunoglobulin superfamily. B7-2 exists predominantly as a monomer on cell surfaces and interacts with two co-stimulatory receptors CD28 and cytotoxic T lymphocyte-associated antigen 4 (CTLA-4) expressed on T cells, and thus induces the signal pathways which regulate T cell activation and tolerance, cytokine production, and the generation of CTL. It is indicated that contacts between B and T helper cells mediated by CD86 encourage signals for the proliferation and IgG secretion of normal B cells and B cell lymphomas. A recent study has revealed that CD86 also promotes the generation of a mature APC repertoire and promotes APC function and survival. CD86 has an important role in chronic hemodialysis, allergic pulmonary inflammation, arthritis, and antiviral responses, and thus is regarded as a promising candidate for immune therapy.

Concentration | O.D. | Average | Corrected ------------------------------------------ 300 pg/ml | 2.231 | 2.273 | 2.191 2.315 150 pg/ml | 1.162 | 1.098 | 1.016 1.034 75 pg/ml | 0.59 | 0.552 | 0.47 0.515 37.5 pg/ml | 0.331 | 0.311 | 0.229 0.292 18.75 pg/ml | 0.213 | 0.207 | 0.125 0.202 9.375 pg/ml | 0.144 | 0.139 | 0.057 0.134 4.6875 pg/ml | 0.099 | 0.106 | 0.024 0.114 0 pg/ml | 0.081 | 0.082 | 0 0.084

Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% ---------------------------------------------------------------- Intra-assay | 1 | 20 | 22.8 | 0.98 | 4.3 Intra-assay | 2 | 20 | 50.6 | 1.5 | 3 Intra-assay | 3 | 20 | 187 | 5.4 | 2.9 Inter-assay | 1 | 20 | 22.7 | 1.8 | 7.8 Inter-assay | 2 | 20 | 50.5 | 3.8 | 7.6 Inter-assay | 3 | 20 | 188 | 10.3 | 5.5

Components | Quantity --------------------- Pre-coated ELISA Plate | 12 x 8 well strips x 1 Standard Solution | 0.2mL x 1 vail Dilution Buffer | 20mL x 1 vail Stop Solution | 6mL x 1 vail Substrate Solution A | 12mL x 1 vail Substrate Solution B | 12mL x 1 vail Wash Buffer Concentrate (25x) | 20mL x 1 vail Detection Antibody Concentrate | 0.6mL x 1 vail