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Human C-C Motif Chemokine 2, CCL2 ELISA Kit

Product Specifications

Background

Monocyte chemoattractant protein 1 (MCP-1), also called CCL2, belongs to a group of CC chemokines located in chromosome 17q11.2. MCP-1 protein interacts with chemokine C-C motif receptor 2 (CCR2) to activate and recruit monocytes, macrophages, CD4+ T cells and immature dendritic cells to the site of infection. The presence of MCP-1 protein in an adequate concentration is important for granuloma formation and M. tuberculosis clearance. Cancer Immunotherapy Immune Checkpoint Immunotherapy Targeted Therapy

Product Name Alternative

C-C motif chemokine 2

Synonyms

MCP1; SCYA2; GDCF2

Gene Name

CCL2

Gene ID

6347

UniProt

P13500

Target

MCP-1

Field of Research

Neuroscience, Cancer, Cardiovascular, Immunology, Metabolism

Assay Type

Sandwich

Assay Protocol

Concentration | O.D. | Average | Corrected ------------------------------------------ 250 pg/ml | 2.549 | 2.466 | 2.335 2.383 125 pg/ml | 1.32 | 1.361 | 1.23 1.402 62.5 pg/ml | 0.823 | 0.793 | 0.662 0.763 31.25 pg/ml | 0.452 | 0.475 | 0.344 0.498 15.625 pg/ml | 0.29 | 0.307 | 0.176 0.324 7.8125 pg/ml | 0.202 | 0.213 | 0.082 0.224 3.90625 pg/ml | 0.165 | 0.175 | 0.044 0.185 0 pg/ml | 0.139 | 0.131 | 0 0.123

Assay Performance Time

3.5h

Sample Type

Serum, plasma, cell culture supernates

Detection Range

3.91-250 pg/mL

Precision

Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% ---------------------------------------------------------------- Intra-assay | 1 | 20 | 18.6 | 1.3 | 7.2 Intra-assay | 2 | 20 | 64.2 | 4.4 | 6.9 Intra-assay | 3 | 20 | 131 | 6.1 | 4.6 Inter-assay | 1 | 20 | 18.4 | 1.9 | 10.1 Inter-assay | 2 | 20 | 63.7 | 6.1 | 9.6 Inter-assay | 3 | 20 | 131 | 10 | 7.7

Sensitivity

0.91 pg/mL

Components

Components | Quantity --------------------- Pre-coated ELISA Plate | 12 x 8 well strips x 1 Standard Solution | 0.2mL x 1 vail Dilution Buffer | 20mL x 1 vail Stop Solution | 6mL x 1 vail Substrate Solution A | 12mL x 1 vail Substrate Solution B | 12mL x 1 vail Wash Buffer Concentrate (25x) | 20mL x 1 vail Detection Antibody Concentrate | 0.6mL x 1 vail

Storage Temperature

2-8°C

Species

Human

Mass

11025

Available Sizes

Frequently Asked Questions

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