Human Tumor Necrosis Factor Receptor Superfamily Member 5, CD40 ELISA Kit

CD40, also known as TNFRSF5, is a member of the TNF receptor superfamily which are single transmembrane-spanning glycoproteins. CD40 protein plays an essential role in mediating a broad variety of immune and inflammatory responses including T cell-dependent immunoglobulin class switching, memory B cell development, and germinal center formation. CD40 protein is expressed in B cells, dendritic cells, macrophages, endothelial cells, and several tumor cell lines. Defects in CD40 result in hyper-IgM immunodeficiency type 3 (HIGM3) . In addition, CD40/CD40L interaction is found to be necessary for amyloid-beta-induced microglial activation, and thus is thought to be an early event in Alzheimer disease pathogenesis. Cancer Immunotherapy Co-stimulatory Immune Checkpoint Targets Immune Checkpoint Immune Checkpoint Detection: Antibodies Immune Checkpoint Detection: ELISA Antibodies Immune Checkpoint Detection: FCM Antibodies Immune Checkpoint Detection: ICC Antibodies Immune Checkpoint Detection: IP Antibodies Immune Checkpoint Detection: WB Antibodies Immune Checkpoint Proteins Immune Checkpoint Targets Immunotherapy Targeted Therapy

Concentration | O.D. | Average | Corrected ------------------------------------------ 250 pg/ml | 1.933 | 1.853 | 1.762 1.774 125 pg/ml | 1.11 | 1.101 | 1.01 1.093 62.5 pg/ml | 0.607 | 0.635 | 0.544 0.664 31.25 pg/ml | 0.392 | 0.378 | 0.287 0.365 15.625 pg/ml | 0.236 | 0.235 | 0.144 0.235 7.8125 pg/ml | 0.176 | 0.164 | 0.073 0.153 3.90625 pg/ml | 0.121 | 0.123 | 0.032 0.126 0 pg/ml | 0.09 | 0.091 | 0 0.093

Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% ---------------------------------------------------------------- Intra-assay | 1 | 20 | 25.1 | 1.2 | 4.6 Intra-assay | 2 | 20 | 61.2 | 2.6 | 4.2 Intra-assay | 3 | 20 | 128 | 6.8 | 5.3 Inter-assay | 1 | 20 | 25.3 | 1.1 | 4.4 Inter-assay | 2 | 20 | 61.1 | 2.7 | 4.3 Inter-assay | 3 | 20 | 128 | 6.7 | 5.2

Components | Quantity --------------------- Pre-coated ELISA Plate | 12 x 8 well strips x 1 Standard Solution | 0.2mL x 1 vail Dilution Buffer | 20mL x 1 vail Stop Solution | 6mL x 1 vail Substrate Solution A | 12mL x 1 vail Substrate Solution B | 12mL x 1 vail Wash Buffer Concentrate (25x) | 20mL x 1 vail Detection Antibody Concentrate | 0.6mL x 1 vail