Human C-X-C Motif Chemokine 5, CXCL5 ELISA Kit

CXCL5 is a small cytokine belonging to the CXC chemokine family. CXC chemokines are particularly significant for leukocyte infiltration in inflammatory diseases. CXCL5 is produced following stimulation of cells with the inflammatory cytokines interleukin-1 or tumor necrosis factor-alpha. It also can be detected in eosinophils, and can be inhibited with the type II interferon. CXCL5 plays a role in reducing sensitivity to sunburn pain in some subjects, and is a potential target which can be utilized to understand more about pain in other inflammatory conditions like arthritis and cystitis. It stimulates the chemotaxis of neutrophils possesses angiogenic properties. It elicits these effects by interacting with the cell surface chemokine receptor CXCR2.

Concentration | O.D. | Average | Corrected ------------------------------------------ 250 pg/ml | 2.274 | 2.304 | 2.179 2.334 125 pg/ml | 1.306 | 1.31 | 1.185 1.314 62.5 pg/ml | 0.775 | 0.742 | 0.617 0.708 31.25 pg/ml | 0.467 | 0.444 | 0.319 0.421 15.625 pg/ml | 0.273 | 0.281 | 0.156 0.288 7.8125 pg/ml | 0.195 | 0.203 | 0.078 0.21 3.90625 pg/ml | 0.172 | 0.163 | 0.038 0.153 0 pg/ml | 0.119 | 0.125 | 0 0.13

Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% ---------------------------------------------------------------- Intra-assay | 1 | 20 | 17.4 | 1.3 | 7.4 Intra-assay | 2 | 20 | 64.6 | 2.7 | 4.2 Intra-assay | 3 | 20 | 166 | 6.7 | 4 Inter-assay | 1 | 20 | 17.5 | 1.2 | 6.8 Inter-assay | 2 | 20 | 64.5 | 4.5 | 7 Inter-assay | 3 | 20 | 166 | 12.2 | 7.3

Components | Quantity --------------------- Pre-coated ELISA Plate | 12 x 8 well strips x 1 Standard Solution | 0.2mL x 1 vail Dilution Buffer | 20mL x 1 vail Stop Solution | 6mL x 1 vail Substrate Solution A | 12mL x 1 vail Substrate Solution B | 12mL x 1 vail Wash Buffer Concentrate (25x) | 20mL x 1 vail Detection Antibody Concentrate | 0.6mL x 1 vail