Human Programmed Cell Death Protein 1, PDCD1 ELISA Kit

Programmed cell death 1, also known as PDCD1, is a type I transmembrane glycoprotein, and is an immunoreceptor belonging to the CD28/CTLA-4 family negatively regulates antigen receptor signaling by recruiting protein tyrosine phosphatase, SHP-2 upon interacting with either of two ligands, PD-L1 or PD-L2. PD1 inhibits the T-cell proliferation and production of related cytokines including IL-1, IL-4, IL-10 and IFN-γ by suppressing the activation and transduction of PI3K/AKT pathway. In addition, coligation of PD1 inhibits BCR-mediating signal by dephosphorylating key signal transducer. PD1 has been suggested to be involved in lymphocyte clonal selection and peripheral tolerance, and thus contributes to the prevention of autoimmune diseases. Furthermore, PD1 is shown to be a regulator of virus-specific CD8+ T cell survival in HIV infection. As a cell surface molecule, PDCD1 regulates the adaptive immune response. Engagement of PD-1 by its ligands PD-L1 or PD-L2 transduces a signal that inhibits T-cell proliferation, cytokine production, and cytolytic function.

Concentration | O.D. | Average | Corrected ------------------------------------------ 250 pg/ml | 2.348 | 2.444 | 2.332 2.539 125 pg/ml | 1.462 | 1.438 | 1.326 1.414 62.5 pg/ml | 0.751 | 0.792 | 0.68 0.833 31.25 pg/ml | 0.466 | 0.468 | 0.356 0.47 15.625 pg/ml | 0.286 | 0.301 | 0.189 0.316 7.8125 pg/ml | 0.209 | 0.199 | 0.087 0.189 3.90625 pg/ml | 0.149 | 0.155 | 0.043 0.161 0 pg/ml | 0.119 | 0.112 | 0 0.105

Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% ---------------------------------------------------------------- Intra-assay | 1 | 20 | 15.2 | 0.61 | 4 Intra-assay | 2 | 20 | 36.2 | 1 | 2.8 Intra-assay | 3 | 20 | 157 | 6.2 | 4 Inter-assay | 1 | 20 | 15.4 | 1.5 | 10 Inter-assay | 2 | 20 | 36.3 | 3.3 | 9.2 Inter-assay | 3 | 20 | 159 | 14.1 | 8.9

Components | Quantity --------------------- Pre-coated ELISA Plate | 12 x 8 well strips x 1 Standard Solution | 0.2mL x 1 vail Dilution Buffer | 20mL x 1 vail Stop Solution | 6mL x 1 vail Substrate Solution A | 12mL x 1 vail Substrate Solution B | 12mL x 1 vail Wash Buffer Concentrate (25x) | 20mL x 1 vail Detection Antibody Concentrate | 0.6mL x 1 vail