Human T-Lymphocyte Activation Antigen Cd86, CD86 ELISA Kit

CD86, also known as B-lymphocyte activation antigen B7-2 (referred to as B70), is a member of the cell surface immunoglobulin superfamily. B7-2 exists predominantly as a monomer on cell surfaces and interacts with two co-stimulatory receptors CD28 and cytotoxic T lymphocyte-associated antigen 4 (CTLA-4) expressed on T cells, and thus induces the signal pathways which regulate T cell activation and tolerance, cytokine production, and the generation of CTL. It is indicated that contacts between B and T helper cells mediated by CD86 encourage signals for the proliferation and IgG secretion of normal B cells and B cell lymphomas. A recent study has revealed that CD86 also promotes the generation of a mature APC repertoire and promotes APC function and survival. CD86 has an important role in chronic hemodialysis, allergic pulmonary inflammation, arthritis, and antiviral responses, and thus is regarded as a promising candidate for immune therapy. Cancer Immunotherapy Co-inhibitory Immune Checkpoint Targets Immune Checkpoint Immune Checkpoint Detection: Antibodies Immune Checkpoint Detection: ELISA Antibodies Immune Checkpoint Detection: FCM Antibodies Immune Checkpoint Detection: IP Antibodies Immune Checkpoint Detection: WB Antibodies Immune Checkpoint Proteins Immune Checkpoint Targets Immunotherapy Targeted Therapy

Concentration | O.D. | Average | Corrected ------------------------------------------ 200 pg/ml | 2.319 | 2.336 | 2.228 2.353 100 pg/ml | 1.307 | 1.307 | 1.199 1.306 50 pg/ml | 0.689 | 0.706 | 0.598 0.722 25 pg/ml | 0.424 | 0.411 | 0.303 0.397 12.5 pg/ml | 0.246 | 0.263 | 0.155 0.279 6.25 pg/ml | 0.21 | 0.197 | 0.089 0.183 3.125 pg/ml | 0.148 | 0.152 | 0.044 0.155 0 pg/ml | 0.113 | 0.108 | 0 0.102

Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% ---------------------------------------------------------------- Intra-assay | 1 | 20 | 10.1 | 0.47 | 4.7 Intra-assay | 2 | 20 | 28.9 | 0.99 | 3.4 Intra-assay | 3 | 20 | 126 | 2.7 | 2.2 Inter-assay | 1 | 20 | 10.1 | 0.42 | 4.2 Inter-assay | 2 | 20 | 28.9 | 0.82 | 2.8 Inter-assay | 3 | 20 | 126 | 3.1 | 2.5

Components | Quantity --------------------- Pre-coated ELISA Plate | 12 x 8 well strips x 1 Standard Solution | 0.2mL x 1 vail Dilution Buffer | 20mL x 1 vail Stop Solution | 6mL x 1 vail Substrate Solution A | 12mL x 1 vail Substrate Solution B | 12mL x 1 vail Wash Buffer Concentrate (25x) | 20mL x 1 vail Detection Antibody Concentrate | 0.6mL x 1 vail