Human Growth-Regulated Alpha Protein, CXCL1 ELISA Kit

The Chemokine (C-X-C motif) Ligand 1, CXCL1, is a small cytokine belonging to the CXC chemokine family that was previously called GRO1 oncogene, GRO?, KC, Neutrophil-activating protein 3 (NAP-3) and melanoma growth stimulating activity, alpha (MSGA-a) . CXCL1 already known to be important in osteoarthritis (OA), as a novel target gene of transcription factor AP-2? in chondrocytes and support the important role of AP-2? in cartilage. CXCL1 is a potent neutrophil chemoattractant with recognized roles in angiogenesis and inflammation. CXCL1 is a novel immediate PTH/PTHrP-responsive gene. CXCL1 may act as a chemoattractant for osteoclast precursors. CXCL1 may also have important pro-nociceptive effects via its direct actions on sensory neurons, and may induce long-term changes that involve protein synthesis. CXCL1 plays a critical nonredundant role in the development of experimental Lyme arthritis and carditis via CXCR2-mediated recruitment of neutrophils into the site of infection. CXCL1 functions through CXCR2 to transactivate the EGFR by proteolytic cleavage of HB-EGF, leading to activation of MAPK signalling and increased proliferation of epithelial ovarian cancer (EOC) cells. It might limit tumor growth by reinforcing senescence early in tumorigenesis. Thus, CXCL1 plays a role in spinal cord development by inhibiting the migration of oligodendrocyte precursors and is involved in the processes of angiogenesis, inflammation, wound healing, and tumorigenesis.

Concentration | O.D. | Average | Corrected ------------------------------------------ 200 pg/ml | 2.583 | 2.501 | 2.386 2.418 100 pg/ml | 1.166 | 1.172 | 1.057 1.177 50 pg/ml | 0.466 | 0.493 | 0.378 0.52 25 pg/ml | 0.258 | 0.262 | 0.147 0.266 12.5 pg/ml | 0.169 | 0.161 | 0.046 0.152 6.25 pg/ml | 0.128 | 0.131 | 0.016 0.134 3.125 pg/ml | 0.119 | 0.117 | 0.002 0.115 0 pg/ml | 0.123 | 0.115 | 0 0.107

Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% ---------------------------------------------------------------- Intra-assay | 1 | 20 | 7.7 | 0.64 | 8.3 Intra-assay | 2 | 20 | 35 | 1.8 | 5.1 Intra-assay | 3 | 20 | 146 | 7.6 | 5.2 Inter-assay | 1 | 20 | 7.8 | 0.55 | 7.1 Inter-assay | 2 | 20 | 34.9 | 2.2 | 6.2 Inter-assay | 3 | 20 | 146 | 7.2 | 4.9

Components | Quantity --------------------- Pre-coated ELISA Plate | 12 x 8 well strips x 1 Standard Solution | 0.2mL x 1 vail Dilution Buffer | 20mL x 1 vail Stop Solution | 6mL x 1 vail Substrate Solution A | 12mL x 1 vail Substrate Solution B | 12mL x 1 vail Wash Buffer Concentrate (25x) | 20mL x 1 vail Detection Antibody Concentrate | 0.6mL x 1 vail