Mouse Phospholipase A2, Pla2g1b ELISA Kit

Phospholipase A2, also known as Phosphatidylcholine 2-acylhydrolase 1B, Group IB phospholipase A2, PLA2 and PLA2G1B, is a secreted protein that belongs to the phospholipase A2 family. Phospholipase A2 / PLA2G1B catalyzes the release of fatty acids from glycero-3-phosphocholines. The best known varieties are the digestive enzymes secreted as zymogens by the pancreas of mammals. Sequences of pancreatic Phospholipase A2 / PLA2G1B enzymes from a variety of mammals have been reported. One striking feature of these enzymes is their close homology to venom phospholipases of snakes. Other forms of Phospholipase A2 / PLA2G1B have been isolated from brain, liver, lung, spleen, intestine, macrophages, leukocytes, erythrocytes, inflammatory exudates, chondrocytes, and platelets. Mice lacking in Phospholipase A2 / PLA2G1B are resistant to obesity and diabetes induced by feeding a diabetogenic high-fat/high-carbohydrate diet. Oral supplementation of a diabetogenic diet with the PLA2G1B inhibitor methyl indoxam effectively suppresses diet-induced obesity and diabetes. PLA2G1B inhibition may be a potentially effective oral therapeutic option for treatment of obesity and diabetes.

Concentration | O.D. | Average | Corrected ------------------------------------------ 200 pg/ml | 2.678 | 2.579 | 2.458 2.479 100 pg/ml | 1.446 | 1.398 | 1.277 1.35 50 pg/ml | 0.777 | 0.788 | 0.667 0.798 25 pg/ml | 0.49 | 0.477 | 0.356 0.464 12.5 pg/ml | 0.292 | 0.299 | 0.178 0.306 6.25 pg/ml | 0.203 | 0.214 | 0.093 0.224 3.125 pg/ml | 0.167 | 0.166 | 0.045 0.165 0 pg/ml | 0.119 | 0.121 | 0 0.123

Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% ---------------------------------------------------------------- Intra-assay | 1 | 20 | 5.8 | 0.51 | 8.7 Intra-assay | 2 | 20 | 29.1 | 2.1 | 7.4 Intra-assay | 3 | 20 | 116 | 9.4 | 8.1 Inter-assay | 1 | 20 | 5.9 | 0.43 | 7.3 Inter-assay | 2 | 20 | 28.8 | 2.3 | 7.9 Inter-assay | 3 | 20 | 117 | 8.1 | 6.9

Components | Quantity --------------------- Pre-coated ELISA Plate | 12 x 8 well strips x 1 Standard Solution | 0.2mL x 1 vail Dilution Buffer | 20mL x 1 vail Stop Solution | 6mL x 1 vail Substrate Solution A | 12mL x 1 vail Substrate Solution B | 12mL x 1 vail Wash Buffer Concentrate (25x) | 20mL x 1 vail Detection Antibody Concentrate | 0.6mL x 1 vail