Human Tumor Necrosis Factor Receptor Superfamily Member 10A, TNFRSF10A ELISA Kit

Tumor necrosis factor receptor superfamily, member 10a (TRAIL R1), also known as TRAIL receptor 1 (TRAIL R1) or CD261 antigen, is a member of the TNF-receptor superfamily. This receptor is activated by tumor necrosis factor-related apoptosis inducing ligand (TNFSF10/TRAIL), and thus transduces cell death signal and induces cell apoptosis. Studies with FADD-deficient mice suggested that FADD, a death domain containing adaptor protein, is required for the apoptosis mediated by this protein. TRAIL R1/CD261/TNFRSF10A serves as a receptor for the cytotoxic ligand TNFSF10/TRAIL. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. TRAIL R1 can promote the activation of NF-kappa-B. TRAIL R1/CD261/TNFRSF10A induces apoptosis of many transformed cell lines but not of normal tissues, even though its death domain-containing receptor, DR4, is expressed on both cell types. Cancer Immunotherapy Immune Checkpoint Immunotherapy Targeted Therapy

Concentration | O.D. | Average | Corrected ------------------------------------------ 150 pg/ml | 2.718 | 2.603 | 2.494 2.487 75 pg/ml | 1.464 | 1.533 | 1.424 1.601 37.5 pg/ml | 0.831 | 0.832 | 0.723 0.832 18.75 pg/ml | 0.479 | 0.458 | 0.349 0.436 9.375 pg/ml | 0.294 | 0.279 | 0.17 0.263 4.6875 pg/ml | 0.188 | 0.198 | 0.089 0.207 2.34375 pg/ml | 0.143 | 0.142 | 0.033 0.14 0 pg/ml | 0.103 | 0.109 | 0 0.114

Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% ---------------------------------------------------------------- Intra-assay | 1 | 20 | 5.1 | 0.16 | 3.2 Intra-assay | 2 | 20 | 17.5 | 0.61 | 3.5 Intra-assay | 3 | 20 | 83.5 | 2.9 | 3.4 Inter-assay | 1 | 20 | 5.1 | 0.33 | 6.4 Inter-assay | 2 | 20 | 17.5 | 1 | 6 Inter-assay | 3 | 20 | 83.8 | 4.5 | 5.4

Components | Quantity --------------------- Pre-coated ELISA Plate | 12 x 8 well strips x 1 Standard Solution | 0.2mL x 1 vail Dilution Buffer | 20mL x 1 vail Stop Solution | 6mL x 1 vail Substrate Solution A | 12mL x 1 vail Substrate Solution B | 12mL x 1 vail Wash Buffer Concentrate (25x) | 20mL x 1 vail Detection Antibody Concentrate | 0.6mL x 1 vail