Human Interleukin-3, IL3 ELISA Kit

IL3 (interleukin 3), also known as IL-3, is a potent growth-promoting cytokine that belongs to the IL-3 family. IL3/IL-3 also belongs to the group of interleukins. Interleukins are produced by a wide variety of body cells. The function of the immune system depends in a large part on interleukins, and rare deficiencies of a number of them have been described, all featuring autoimmune diseases or immune deficiency. The majority of interleukins are synthesized by helper CD4+ T lymphocytes, as well as through monocytes, macrophages, and endothelial cells. They promote the development and differentiation of T, B, and hematopoietic cells. IL3/IL-3 is capable of supporting the proliferation of a broad range of hematopoietic cell types. It is involved in a variety of cell activities such as cell growth, differentiation, and apoptosis. IL3/IL-3 has been shown to also possess neurotrophic activity, and it may be associated with neurologic disorders.

Concentration | O.D. | Average | Corrected ------------------------------------------ 150 pg/ml | 2.39 | 2.476 | 2.389 2.562 75 pg/ml | 1.335 | 1.393 | 1.306 1.451 37.5 pg/ml | 0.739 | 0.746 | 0.659 0.753 18.75 pg/ml | 0.427 | 0.423 | 0.336 0.419 9.375 pg/ml | 0.258 | 0.252 | 0.165 0.245 4.6875 pg/ml | 0.163 | 0.172 | 0.085 0.18 2.34375 pg/ml | 0.126 | 0.133 | 0.046 0.14 0 pg/ml | 0.083 | 0.087 | 0 0.091

Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% ---------------------------------------------------------------- Intra-assay | 1 | 20 | 10.2 | 0.29 | 2.9 Intra-assay | 2 | 20 | 32.9 | 1.2 | 3.6 Intra-assay | 3 | 20 | 84.4 | 2.2 | 2.6 Inter-assay | 1 | 20 | 10.3 | 0.39 | 3.8 Inter-assay | 2 | 20 | 33.3 | 2.5 | 7.5 Inter-assay | 3 | 20 | 84.2 | 3.2 | 3.7

Components | Quantity --------------------- Pre-coated ELISA Plate | 12 x 8 well strips x 1 Standard Solution | 0.2mL x 1 vail Dilution Buffer | 20mL x 1 vail Stop Solution | 6mL x 1 vail Substrate Solution A | 12mL x 1 vail Substrate Solution B | 12mL x 1 vail Wash Buffer Concentrate (25x) | 20mL x 1 vail Detection Antibody Concentrate | 0.6mL x 1 vail