Human Beta-Nerve Growth Factor, NGF ELISA Kit

Nerve growth factor (NGF) is important for the development and maintenance of the sympathetic and sensory nervous systems. NGF protein was identified as a large complex consisting of three non-covalently linked subunits, α, β, and γ, among which, the β subunit, called β-NGF (beta-NGF), was demonstrated to exhibits the growth-stimulating activity of NGF protein. NGFB/beta-NGF gene is a member of the NGF-beta family and encodes a secreted protein that homodimerizes and is incorporated into a larger complex. NGF protein acts via at least two receptors on the surface of cells (TrkA and p75 receptors) to regulate neuronal survival, promote neurite outgrowth, and up-regulate certain neuronal functions such as mediation of pain and inflammation. Also, previous studies indicated that NGF may also have an important role in the regulation of the immune system.

Concentration | O.D. | Average | Corrected ------------------------------------------ 140 pg/ml | 2.254 | 2.243 | 2.125 2.232 70 pg/ml | 1.213 | 1.282 | 1.164 1.35 35 pg/ml | 0.679 | 0.709 | 0.591 0.739 17.5 pg/ml | 0.45 | 0.427 | 0.309 0.404 8.75 pg/ml | 0.274 | 0.275 | 0.157 0.276 4.375 pg/ml | 0.19 | 0.197 | 0.079 0.203 2.1875 pg/ml | 0.165 | 0.158 | 0.04 0.151 0 pg/ml | 0.123 | 0.118 | 0 0.113

Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% ---------------------------------------------------------------- Intra-assay | 1 | 20 | 9.5 | 0.65 | 6.8 Intra-assay | 2 | 20 | 27.9 | 1.3 | 4.5 Intra-assay | 3 | 20 | 111 | 4.4 | 4 Inter-assay | 1 | 20 | 9.6 | 0.86 | 9 Inter-assay | 2 | 20 | 27.8 | 1.5 | 5.3 Inter-assay | 3 | 20 | 110 | 7.7 | 7

Components | Quantity --------------------- Pre-coated ELISA Plate | 12 x 8 well strips x 1 Standard Solution | 0.2mL x 1 vail Dilution Buffer | 20mL x 1 vail Stop Solution | 6mL x 1 vail Substrate Solution A | 12mL x 1 vail Substrate Solution B | 12mL x 1 vail Wash Buffer Concentrate (25x) | 20mL x 1 vail Detection Antibody Concentrate | 0.6mL x 1 vail