Human Interleukin-33, IL33 ELISA Kit

Interleukin 33 (IL-33), also known as DVS27 or NF-HEV (Nuclear Factor from High Endothelial Venules), is a pro-inflammatory protein and a chromatin-associated cytokine of the IL-1 family with high sequence and structural similarity to IL-1 and IL-18. IL-33 protein is expressed highly and rather selectively by high endothelial venule endothelial cells (HEVECs) in human tonsils, Peyer's patches, and lymph nodes. IL-33 protein has transcriptional regulatory properties, and the researches suggested that IL-33 is a dual-function protein that might act both as a cytokine and as an intracellular nuclear factor. As a type 2 cytokines, IL-33 protein also play a pivotal role in helminthic infection and allergic disorders.

Concentration | O.D. | Average | Corrected ------------------------------------------ 100 pg/ml | 2.339 | 2.308 | 2.199 2.278 50 pg/ml | 1.342 | 1.365 | 1.256 1.388 25 pg/ml | 0.744 | 0.768 | 0.659 0.792 12.5 pg/ml | 0.452 | 0.439 | 0.33 0.426 6.25 pg/ml | 0.253 | 0.27 | 0.161 0.288 3.125 pg/ml | 0.174 | 0.183 | 0.074 0.193 1.5625 pg/ml | 0.156 | 0.15 | 0.041 0.145 0 pg/ml | 0.106 | 0.109 | 0 0.113

Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% ---------------------------------------------------------------- Intra-assay | 1 | 20 | 6.5 | 0.18 | 2.8 Intra-assay | 2 | 20 | 32.6 | 1.3 | 3.9 Intra-assay | 3 | 20 | 65.2 | 3.3 | 5 Inter-assay | 1 | 20 | 6.4 | 0.46 | 7.2 Inter-assay | 2 | 20 | 32.4 | 2 | 6.1 Inter-assay | 3 | 20 | 65.5 | 4.7 | 7.2

Components | Quantity --------------------- Pre-coated ELISA Plate | 12 x 8 well strips x 1 Standard Solution | 0.2mL x 1 vail Dilution Buffer | 20mL x 1 vail Stop Solution | 6mL x 1 vail Substrate Solution A | 12mL x 1 vail Substrate Solution B | 12mL x 1 vail Wash Buffer Concentrate (25x) | 20mL x 1 vail Detection Antibody Concentrate | 0.6mL x 1 vail