Human Interleukin-17A, IL17A ELISA Kit

IL17, also known as IL17a, is a cytokine that belongs to the IL-17 family. Cytokines are proteinaceous signaling compounds that are major mediators of the immune response. They control many different cellular functions including proliferation, differentiation, and cell survival/apoptosis but are also involved in several pathophysiological processes including viral infections and autoimmune diseases. Cytokines are synthesized under various stimuli by a variety of cells of both the innate (monocytes, macrophages, dendritic cells) and adaptive (T- and B-cells) immune systems. The IL-17 family of cytokines includes six members, IL-17/IL-17A, IL-17B, IL-17C, IL-17D, IL-17E/IL-25, and IL-17F, which are produced by multiple cell types. IL-17 regulates the activities of NF-kappaB and mitogen-activated protein kinases. This cytokine can stimulate the expression of IL6 and cyclooxygenase-2 (PTGS2/COX-2), as well as enhance the production of nitric oxide (NO) . High levels of IL-17 are associated with several chronic inflammatory diseases including rheumatoid arthritis, psoriasis, and multiple sclerosis. Cancer Immunotherapy Immune Checkpoint Immunotherapy Targeted Therapy

Concentration | O.D. | Average | Corrected ------------------------------------------ 75 pg/ml | 2.565 | 2.599 | 2.465 2.633 37.5 pg/ml | 1.262 | 1.331 | 1.197 1.401 18.75 pg/ml | 0.765 | 0.741 | 0.607 0.718 9.375 pg/ml | 0.432 | 0.435 | 0.301 0.439 4.6875 pg/ml | 0.285 | 0.283 | 0.149 0.281 2.34375 pg/ml | 0.189 | 0.199 | 0.065 0.21 1.171875 pg/ml | 0.166 | 0.163 | 0.029 0.161 0 pg/ml | 0.14 | 0.134 | 0 0.129

Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% ---------------------------------------------------------------- Intra-assay | 1 | 20 | 5.6 | 0.18 | 3.3 Intra-assay | 2 | 20 | 17.1 | 0.49 | 2.9 Intra-assay | 3 | 20 | 40.7 | 0.92 | 2.3 Inter-assay | 1 | 20 | 5.6 | 0.36 | 6.4 Inter-assay | 2 | 20 | 17.1 | 0.8 | 4.7 Inter-assay | 3 | 20 | 40.4 | 1.9 | 4.8

Components | Quantity --------------------- Pre-coated ELISA Plate | 12 x 8 well strips x 1 Standard Solution | 0.2mL x 1 vail Dilution Buffer | 20mL x 1 vail Stop Solution | 6mL x 1 vail Substrate Solution A | 12mL x 1 vail Substrate Solution B | 12mL x 1 vail Wash Buffer Concentrate (25x) | 20mL x 1 vail Detection Antibody Concentrate | 0.6mL x 1 vail