Rat Cd59 Glycoprotein, Cd59 ELISA Kit

CD59 glycoprotein, also known as 2 kDa homologous restriction factor, HRF2, MAC-inhibitory protein, Membrane attack complex inhibition factor, Membrane inhibitor of reactive lysis, MIC11, MIRL and CD59, is a cell membrane protein which contains one UPAR/Ly6 domain. CD59 is a small, highly glycosylated, GPI-linked protein, with a wide expression profile. The soluble form of CD59 from urine retains its specific complement binding activity, but exhibits greatly reduced ability to inhibit MAC assembly on cell membranes. CD59 is a potent inhibitor of the complement membrane attack complex (MAC) action. CD59 was first identified as a regulator of the terminal pathway of complement. It acts by binding to the C8 and/or C9 complements of the assembling MAC, thereby preventing incorporation of the multiple copies of C9 required for complete formation of the osmolytic pore. This inhibitor appears to be species-specific. CD59 is involved in signal transduction for T-cell activation complexed to a protein tyrosine kinase. Defects in CD59 are the cause of CD59 deficiency (CD59D) .

Concentration | O.D. | Average | Corrected ------------------------------------------ 70 pg/ml | 2.838 | 2.729 | 2.605 2.62 35 pg/ml | 1.545 | 1.535 | 1.411 1.525 17.5 pg/ml | 0.842 | 0.863 | 0.739 0.884 8.75 pg/ml | 0.487 | 0.515 | 0.391 0.542 4.375 pg/ml | 0.3 | 0.314 | 0.19 0.328 2.1875 pg/ml | 0.224 | 0.221 | 0.097 0.218 1.09375 pg/ml | 0.162 | 0.172 | 0.048 0.182 0 pg/ml | 0.119 | 0.124 | 0 0.129

Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% ---------------------------------------------------------------- Intra-assay | 1 | 20 | 7 | 0.21 | 3 Intra-assay | 2 | 20 | 17 | 0.72 | 4.3 Intra-assay | 3 | 20 | 42.5 | 1.4 | 3.4 Inter-assay | 1 | 20 | 7 | 0.64 | 9.2 Inter-assay | 2 | 20 | 16.9 | 1.1 | 6.6 Inter-assay | 3 | 20 | 42.2 | 3.5 | 8.4

Components | Quantity --------------------- Pre-coated ELISA Plate | 12 x 8 well strips x 1 Standard Solution | 0.2mL x 1 vail Dilution Buffer | 20mL x 1 vail Stop Solution | 6mL x 1 vail Substrate Solution A | 12mL x 1 vail Substrate Solution B | 12mL x 1 vail Wash Buffer Concentrate (25x) | 20mL x 1 vail Detection Antibody Concentrate | 0.6mL x 1 vail