H2DCFDA (DCFH-DA)
Product Specifications
CAS Number
4091-99-0
Synonyms
2',7'-Dichlorodihydrofluorescein diacetate
Target
ROS
Applications
NF-κB
Smiles
CC (=O) OC1=CC2=C (C=C1Cl) C (C3=C (O2) C=C (OC (C) =O) C (=C3) Cl) C4=C (C=CC=C4) C (O) =O
Molecular Formula
C24H16Cl2O7
Additionnal Information
H2DCFDA (DCFH-DA) is a cell-permeable probe used to detect intracellular reactive oxygen species (ROS) production. It is de-esterified intracellularly and turns to highly fluorescent 2′,7′-dichlorofluorescein upon oxidation. It is used in sensitive and rapid quantitation of oxygen-reactive species in response to oxidative metabolism; microplate assay for detecting oxidative products in phagocytic cells, and quantitative multiwell myeloid differentiation assay. (Ex/Em=488/525 nm)
Storage Conditions
3 years -20°C (in the dark) powder
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