Human Granzyme B Activity Detection Substrate for Flow Cytometry

Human Granzyme B Substrate (Green) is generated by conjugating a Granzyme B-specific recognition peptide (sequence IEPD) with a high-affinity DNA fluorescent dye. The substrate is cell-membrane-permeable and can penetrate the plasma membrane into the cytoplasm. In living cells without active Granzyme B, charge repulsion occurs between the substrate and DNA, resulting in no cellular fluorescence signal. In contrast, in living cells with active Granzyme B, the activated Granzyme B cleaves the substrate and releases the high-affinity DNA dye. The released DNA dye binds to intracellular DNA and emits green fluorescence, thereby enabling the detection of Granzyme B activity.