Dihydroorotate Dehydrogenase (DHODH) Activity Fluorometric Assay Kit
Product Specifications
Abbreviation
DHODH
Field of Research
Metabolic Diseases
Detection Method
Fluorometric method
Assay Principle
Dihydroorotate Dehydrogenase (DHODH) is a key enzyme in the de novo synthesis pathway of pyrimidine nucleotides. DHODH catalyzes the formation of orotic acid and reacts with a chromogenic agent to produce a fluorescent substance. The fluorescence intensity is positively correlated with the activity of DHODH.
Assay Performance Time
1 h 30 min
Sample Type
Animal tissue; Cell
Detection Range
0.008-2.5 U/L
Sensitivity
0.008 U/L
Shipping Conditions
Ice bag
Storage Conditions
This product can be stored at -20°C for 12 months with shading light.
Inter CV
6.6%-8.6%
Intra CV
2.2%-5.9%
Detection Instrument
Fluorescence microplate reader
Result Type
Enzyme activity
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