CellaQuantTM Human KIM-1 (Kidney Injury Molecule 1) ELISA Kit

TIM-1 (T-cell immunoglobulin and mucin domain protein 1; also known as KIM-1 and HAVCR) is a 70–80 kDa type I transmembrane glycoprotein belonging to the TIM immunoglobulin superfamily (1–4) . This family regulates the balance between Th1 and Th2 immune responses. Structurally, TIM proteins contain an N-terminal immunoglobulin variable (IgV) domain, a glycosylated mucin domain, a transmembrane region, and a cytoplasmic tail.TIM proteins recognize phosphatidylserine exposed on apoptotic cells via a metal-ion-dependent binding pocket within the IgV domain. Notably, mouse TIM-2 lacks this pocket and does not bind phosphatidylserine. TIM-1 also interacts with P-selectin (CD62P) with higher affinity than with E- or L-selectin, mediating T-cell trafficking and costimulation. Studies using a contact hypersensitivity model indicate that TIM-1–P-selectin interaction facilitates the migration of activated T cells to inflamed skin.

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human KIM-1. Samples (or Standards &Quality Control Material) are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human KIM-1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human KIM-1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450±2 nm. The OD value is proportional to the concentration of Human KIM-1. You can calculate the concentration of Human KIM-1 in the samples by comparing the OD of the samples to the standard curve.

This ELISA kit applies to the in vitro quantitative determination of Human KIM-1 concentrations in Cell culture supernatant, Cell lysates or other biological fluids.