Hieff NGS DNA Spike in Mix for CUT&Tag

Hieff NGS DNA Spike-in Mix for CUT&Tag (5 ng/μL) is derived from three sequences of Lambda DNA from E. coli, with lengths of 230 bp, 250 bp, and 300 bp, and a molar concentration ratio of approximately 1:3:10. This standard is primarily used for normalization and quantitative analysis of CUT&Tag sequencing data under different treatment conditions or varying cell states. Add 1 μL per 100,000 cells, with adjustments possible based on the binding capacity and abundance of the target protein.