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Recombinant Murine Interleukin-3

Product Specifications

Background

Interleukin-3 (IL-3) is a type of biological signal (cytokine) which is encoded by the IL-3 gene located on chromosome 5 and produced primarily by activated T cells beside human thymic epithelial cells, activated murine mast cells, murine keratinocytes and neurons/astrocytes. The protein acts in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. In addition, it exerts its biological activities through binding to interleukin-3 receptors included α and β subunits. The Mouse IL-3 is different from human IL-3 and contains 140 amino acids residues. Specifically, mouse and human IL-3 share low homology and have not cross species activity.

Product Name Alternative

Hematopoietic growth factor, MCGF, Multipotential colony-stimulating factor, P-cell-stimulating factor.

Gene ID

16187

UniProt

P01586

Target

DTHRLTRTLN CSSIVKEIIG KLPEPELKTD DEGPSLRNKS FRRVNLSKFV ESQGEVDPED RYVIKSNLQK LNCCLPTSAN DSALPGVFIR DLDDFRKKLR FYMVHLNDLE TVLTSRPPQP ASGSVSPNRG TVEC

Purification

> 98 % by SDS-PAGE and HPLC analyses.

Endotoxin

Less than 1 EU/ug of rMuIL-3 as determined by LAL method.

Bioactivity

7

Storage Conditions

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculated Molecular Weight

Approximately 14.8 kDa globular protein containing 134 amino acid residues.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS, pH 7.4.

Host or Source

Escherichia coli

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