Mouse C3 Antibody (C3/C3a)
A rat monoclonal antibody to an epitope on mouse C3 protein.
Product Specifications
Background
The human complement component, C3, consists of two disulfide-bonded subunits (Alpha – 115,000 kD and Beta – 75,000 kD) . The concentration of C3 in serum is approximately 1.25 + 0.52 mg/mL. Under normal conditions, activation of either the Classical, Lectin or Alternative Complement Pathways produces C3 convertase which cleaves C3 into two fragments – C3a, an anaphylatoxin, and C3b.
Cross Reactivity
None
Immunogen
Recombinant mouse C3 protein immunized in rat.
Clonality
Monoclonal
Source
Rat
Applications
ELISA, WB (See citations and technical data sheet for application info)
Concentration
~1 mg/mL. Varies by lot so refer to CoA.
Purity
≥ 85% by SDS PAGE
Form
Liquid (PBS, pH 7.2)
Storage Conditions
Short term (30 days) 4 ̊C. Long term at or below –20 ̊C.
Specificity
The specificity of the monoclonal antibody was established via a series of immunoassays. The antibody was shown by ELISA and Western Blot (WB) to bind highly purified mouse intact C3 and C3a fragment protein, but not C3b, iC3b, C3dg, or C3d.
Isotype
IgG2aκ
Conjugate
Unconjugated
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