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MICROVUETMRat PTH ELISA

The MICROVUE Rat Intact PTH ELISA Kit is for the measurement of PTH in rat serum, plasma or cell culture media.

Product Specifications

Background

Rat intact parathyroid hormone (PTH) is an 84 amino acid polypeptide produced by the parathyroid gland with its biological activity residing in the N-terminal region of the peptide. PTH plays an important role in maintaining the concentration of ionized calcium within the limits needed to achieve normal metabolic functions. When serum calcium levels are decreased the parathyroid gland increases secretion of the hormone which results in increased mobilization of calcium from skeletal reserves into the circulation. When levels of serum calcium are increased the secretion of PTH is reduced.

Cross Reactivity

Cross-reactivity with human PTH 1-84 is 2.3%.

Assay Performance Time

3.5 hours

Standard

6

Sample Type

Plasma, Serum, or Cell Culture Supernatent, 50 μL

Limit Of Detection

0.002 ng/mL

Form

96 well plate with 12 eight-well strips in a resealable foil pouch

Storage Conditions

Store unopened kit between 2°C to 8°C. Refer to manual for storing individual components

Controls

2

Inter Assay

5.1 - 6.0%

Intra Assay

2.1 - 2.4%

Frequently Asked Questions

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