MICROVUETMRat PTH ELISA
The MICROVUE Rat Intact PTH ELISA Kit is for the measurement of PTH in rat serum, plasma or cell culture media.
Product Specifications
Background
Rat intact parathyroid hormone (PTH) is an 84 amino acid polypeptide produced by the parathyroid gland with its biological activity residing in the N-terminal region of the peptide. PTH plays an important role in maintaining the concentration of ionized calcium within the limits needed to achieve normal metabolic functions. When serum calcium levels are decreased the parathyroid gland increases secretion of the hormone which results in increased mobilization of calcium from skeletal reserves into the circulation. When levels of serum calcium are increased the secretion of PTH is reduced.
Cross Reactivity
Cross-reactivity with human PTH 1-84 is 2.3%.
Assay Performance Time
3.5 hours
Standard
6
Sample Type
Plasma, Serum, or Cell Culture Supernatent, 50 μL
Limit Of Detection
0.002 ng/mL
Form
96 well plate with 12 eight-well strips in a resealable foil pouch
Storage Conditions
Store unopened kit between 2°C to 8°C. Refer to manual for storing individual components
Controls
2
Inter Assay
5.1 - 6.0%
Intra Assay
2.1 - 2.4%
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