Factor H Depleted Sera
Normal human serum depleted of complement Factor H protein.
Product Specifications
Background
Normal human serum was depleted of factor H by immunoaffinity chromatography. The product is tested for the absence of factor H by double immunodiffusion. Factor H is a regulator of complement activation. As a result, factor H-Dpl is still capable of activating the alternative pathway and does so spontaneously and very rapidly in the absence of factor H. In fact, activation occurs in less than 2 min in the fluid phase without an activating particle resulting in the consumption of both C3 and factor B. For this reason this depleted serum is stored with 0.1 mM EDTA to inhibit spontaneous activation.
Source
Normal human serum
Applications
See citations and technical data sheet for application info.
Concentration
>50 mg protein/mL (refer to CoA for lot specific concentration) .
Purity
No Factor H detectable by immunodiffusion.
Activity
>80% versus normal human serum standard.
Form
Frozen liquid. 10 mM sodium phosphate, 145 mM NaCl, pH 7.3. No preservative, 0.22 μm filtered.
Storage Conditions
Store at -70°C or below. Minimize freeze/thaw cycles.
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