Factor H Antibody (Polyclonal)

Factor H is a single-chain glycoprotein with a molecular weight of approximately 150 KD. The concentration of this protein in normal human plasma/serum is approximately 500 μg/mL. Factor H can regulate the complement system on both the cellular surface and in fluid phase, and plays roles in both the alternative and classical pathways of activation. Factor H regulates formation and function of C3 convertase enzyme in the alternative pathway. It binds to C3b and inhibits C3 convertase formation, dissociates assembled C3 convertase, accelerates Factor I mediated cleavage of C3b to C3bi, and hinders C5 binding to the C3b subcomponent of both the alternative and classical pathways. Factor H also regulates the spontaneous fluid-phase activation of the alternative complement pathway by C3b-like forms of C3 that continuously arise in plasma and serum. Therefore, when concentrations of Factor H fall below normal levels, rapid fluid-phase activation occurs which causes consumption of complement components both in vivo and in vitro.

The Anti-human Factor H polyclonal antisera was tested against normal human serum/plasma by immunodiffusion, one-dimensional immunoelectrophoresis, and rocket immunoelectrophoresis. The antisera was determined to be monospecific for Factor H at varying concentrations.