C9 Depleted Sera
Normal human serum depleted of complement C9 protein.
Product Specifications
Background
Normal human serum depleted of complement C9 protein by immunoaffinity chromatography. The product is tested for the absence of C9 by functional assays for classical pathway activity and alternative pathway activity and for C9 protein by double immunodiffusion. Note that the C5b678 complex has considerable hemolytic potential without C9 and therefore C9-Dpl is capable of lysing EA and most other cells directly without added C9 if activation is strong enough and if the concentration of depleted serum used is high enough.
Source
Normal human serum
Applications
See citations and technical data sheet for application info.
Concentration
>50 mg protein/mL (refer to CoA for lot specific concentration) .
Purity
No C9 detectable by immunodiffusion.
Activity
>80% versus normal human serum standard.
Form
Frozen liquid. 10 mM sodium phosphate, 145 mM NaCl, pH 7.3. No preservative, 0.22 μm filtered.
Storage Conditions
Store at -70°C or below. Minimize freeze/thaw cycles.
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