C5 Protein

Purified human C5 protein

Product Specifications

Background

Purified human complement component C5. Complement component C5 is unique to the terminal complement pathway. 190 kD C5 protein is comprised of two chains (α 115 kD; ß 75 kD) and is present in plasma at 70 mg/L. Activation of either complement pathway can generate a C5 convertase enzyme capable of cleaving C5 to C5a (a potent anaphylatoxin) and C5b. Together with the other proteins of the terminal pathway, C5b forms the membrane attack complex or MAC.

Source

Normal human serum

Applications

See citations and technical data sheet for application info.

Concentration

1.0 mg/mL (refer to CoA for lot specific concentration)

Purity

>90% by SDS-PAGE

Activity

>80% versus normal human serum standard.

Form

Frozen liquid. 10 mM sodium phosphate, 145 mM NaCl, pH 7.2. No preservative, 0.22 μm filtered.

Molecular Weight

190,000 Da (2 chains)

Storage Conditions

Store at -70°C or below. Avoid freeze/thaw.