C3b Protein
Purified human C3b protein
Product Specifications
Background
C3b is derived from native C3 upon cleavage and release of C3a. It is prepared by cleavage with the alternative pathway C3 convertase. C3b is central to the function of all three pathways of complement. Initiation of each pathway generates proteolytic enzyme complexes (C3 convertases) which are bound to the target surface. These enzymes cleave a peptide bond in C3 releasing the anaphylatoxin C3a and activating C3b. For a brief time (~60 μs) this nascent C3b is capable of reacting with and covalently coupling to hydroxyl groups on the target surface. Carbohydrates are the favored target, but protein hydroxyls and amino groups also react. This process of tagging the target surface with C3b is called opsonization.
Source
Normal human serum
Applications
See citations and technical data sheet for application info.
Concentration
1.0 mg/mL (refer to CoA for lot specific concentration)
Purity
>90% by SDS-PAGE
Activity
No activity
Form
Frozen liquid. 10 mM sodium phosphate, 145 mM NaCl, pH 7.2. No preservative, 0.22 μm filtered.
Molecular Weight
176,000 Da (2 chains)
Storage Conditions
Store at -70°C or below. Avoid freeze/thaw.
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