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C3 Protein

Purified human C3 protein

Product Specifications

Background

Purified human complement component C3. The third component of complement is a centrally important constituent of both the alternative and classical pathways. Upon secretion, pro-C3 undergoes a site specific proteolytic cleavage to yield circulating C3, a protein containing two disulfide bonded subunits (Alpha 115 kD and Beta 75 kD) . The concentration of C3 in serum is approximately 1.25 +/– 0.52 mg/mL.

Source

Normal human serum

Applications

See citations and technical data sheet for application info.

Concentration

1.0 mg/mL (refer to CoA for lot specific concentration)

Purity

>85% by SDS-PAGE

Activity

>70% versus normal human serum standard

Form

Frozen liquid. 10 mM sodium phosphate, 145 mM NaCl, pH 7.2. No preservative, 0.22 μm filtered.

Molecular Weight

185,000 Da (2 chains)

Storage Conditions

Store at -70°C or below. Avoid freeze/thaw (>10 % activity loss per thaw) .

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