MICROVUETMTRAP5b EIA
The MICROVUE TRAP5b Assay is an immunocapture enzyme assay for the determination of tartrate-resistant acid phosphatase isoform 5b (TRAcP 5b) in human serum and plasma.
Product Specifications
Background
TRAP5b (serum band 5 tartrate-resistant acid phosphatase, TRAcP 5b; EC 3.1.3.2) is a 35-37 kDa glycoprotein. TRAP5b is typically expressed in proportion to osteoclast activity and is secreted into circulation. As a result, serum TRAP5b is considered a potentially useful marker for bone resorption. The MICROVUE TRAP5b EIA detects the enzyme activity of bound TRAP5b in experimental samples.
Cross Reactivity
None
Assay Performance Time
2 hours
Standard
5
Sample Type
Serum/Heparin Plasma 50 μL
Sample Volume
Females (Ages 30–44) (premenopausal) 2.9±1.4 U/L or (Ages ≥50) (postmenopausal) 4.3±1.5 U/L, Males (Ages ≥20) 4.0±1.4 U/L
Limit Of Detection
0.24 U/L
Form
96 well plate with murine monoclonal anti-TRAP5b antibodies
Storage Conditions
Store unopened kit between 2°C to 8°C. Refer to manual for storing individual components
Controls
2
Inter Assay
2.0–3.0%
Intra Assay
1.9–2.2%
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