MICROVUETMOsteocalcin EIA
The MICROVUE Osteocalcin immunoassay measures intact (de novo) osteocalcin in serum.
Product Specifications
Background
Osteocalcin (OC) or BGP (bone gla protein) is found exclusively in bone tissue. It is a 5800 molecular weight extrahepatic vitamin K dependent protein produced by osteoblasts. It contains three gamma-carboxyglutamic acid residues which are thought to be involved in calcium ion and hydroxyapatite binding. It accounts for 10%-20% of the noncollagenous protein in bone. While the in vivo function of osteocalcin is unknown, its affinity for bone mineral constituents implies a role in bone formation. A Highly Specific Assay for Intact OC 1) Does not detect carboxy- or aminoterminal peptides, 2) Requires tertiary structure for antibody recognition, and 3) Detects intact OC, not OC fragments released during osteoclastic resorption.
Cross Reactivity
Cow, Cynomolgous monkey, Guinea Pig, Horse, Goat, Pig, Rabbit, Sheep
Assay Performance Time
3.6 hours
Standard
6
Sample Type
Serum 25 μL
Sample Volume
Adult: 3.7–10.0 ng/mL
Limit Of Detection
0.45 ng/mL
Form
96 well plate with 12 eight-well strips in a resealable foil pouch
Storage Conditions
Store unopened kit between 2°C to 8°C. Refer to manual for storing individual components
Controls
2
Inter Assay
4.8–9.8%
Intra Assay
4.8–10.0%
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