Microtome Price

Xiaogan Kuohai Medical Technology Co., Ltd. is a rare domestic pathology instrument manufacturing company with a widespread user base throughout China. "Kuohai Medical Technology" owns five subsidiaries, including Hubei Xiaogan Kuohai Medical Technology Co., Ltd., Xiaogan Kuohai Medical Technology Co., Ltd., Hubei Haishi Industrial Co., Ltd., Xiaogan Ruifeng Electronic Technology Co., Ltd., and Xiaogan Dinghang Decoration Engineering Co., Ltd. The product range covers industries such as medical instruments, biotechnology, electronic technology, high-end building materials, and decoration engineering. The company is steadily moving towards industrial group development. Rich Experience Xiaogan Kuohai Medical Technology Co., Ltd. is a rare domestic pathology instrument manufacturing company with a widespread user base throughout China. High Quality The product range covers industries such as medical instruments, biotechnology, electronic technology, high-end building materials, and decoration engineering. Technical Support Our team of experts is available to assist with troubleshooting, answer technical inquiries, and provide guidance. Customer Service We prioritize open communication to address our clients' specific requirements and deliver personalized solutions. Semi-Automatic Microtome A semi-automatic microtome is a laboratory instrument designed to cut thin sections of various materials, particularly biological tissues, for microscopic examination. Unlike a fully manual microtome, which requires the user to perform all movements to advance the sample and the knife, a semi-automatic microtome automates some functions to make the process more efficient and repeatable. Freezing Microtome A freezing microtome, commonly referred to as a cryostat, is a laboratory instrument used to cut thin sections of frozen biological tissues for microscopic analysis. The cryostat maintains the tissue at a consistently low temperature, usually below -20°C, which keeps the specimen firm and minimizes the formation of ice crystals that could disrupt cellular structures. Automatic Microtome An automatic microtome is a laboratory instrument used in histology and pathology to produce thin, uniform sections of biological tissues for microscopic examination. The device automates the process of cutting tissue blocks into slices, which are typically 4-10 micrometers thick, allowing for detailed analysis under a microscope. Tissue Microtome A tissue microtome is a specialized piece of laboratory equipment used in histology and pathology to produce thin sections of biological tissues for microscopic examination. The primary purpose of using a microtome is to create sections that are thin enough-typically between 4 and 10 micrometers-to allow light to pass through during microscopy, enabling the observation of cellular structures and details. Rotary Microtome A rotary microtome is a laboratory instrument used to cut thin sections of biological tissues for microscopic examination. Unlike linear microtomes that cut sections in a back-and-forth motion, rotary microtomes cut sections in a continuous circular motion. Frozen Microtome A frozen microtome is a specialized type of microtome designed specifically for cutting thin sections of biological tissues that have been flash-frozen. The process involves rapidly cooling the tissue to a very low temperature, usually by immersing it in liquid nitrogen or another cryogenic fluid, which makes it rigid and easier to cut. What is Semi-Automatic Microtome A semi-automated microtome uses a specimen feed that is motorized, but the rotary motion of the handwheel is still done manually and offers a medium throughput. The semi-automated method works well for thick and hard sections, using a consistent force when cutting through a block. Automating the specimen feed provides the precision of a motorized specimen feeding that yields consistent, reproducible sections.Semi-Automatic Microtome is mainly used for slicing thin sections of different specimens, as biopsies for pathological diagnosis, tissue samples of animal and plant, plastic, cork, wood, polystyrene, etc. This microtome has stable performance, easy operation and exemplary comfort; as well as ergonomic design and handwheel slight, which greatly reduces intensity of job. Precision and Consistency One of the primary advantages of using a semi-automatic microtome is the ability to achieve highly precise and consistent sections. The instrument's motorized advance and retract functions, coupled with its programmable cutting speed and thickness, enable pathologists and researchers to obtain sections of a desired thickness with minimal variation. This precise sectioning plays a crucial role in accurate diagnosis and subsequent treatment decisions. Increased Efficiency and Productivity The semi-automatic microtome significantly reduces the time and effort involved in sectioning tissues compared to manual microtomes. With automated functioning, it eliminates the need for repetitive manual adjustments, allowing technicians to process more samples in a shorter span of time. This enhanced efficiency not only increases laboratory productivity but also enables quicker turnaround times for diagnosis and research studies. Safety Features The safety of laboratory personnel is paramount, and the semi-automatic microtome takes this aspect into consideration. Equipped with safety features such as blade guards and sensor-controlled movements, it minimizes the risk of accidents or injuries during operation. These safety measures ensure that users can work with confidence, focusing on their tasks without compromising their well-being. Versatility and Adaptability Another noteworthy feature of the semi-automatic microtome is its versatility. It offers various options for specimen orientation, adjustable cutting angles, and interchangeable knives, allowing users to cater to different tissue types and experimental requirements. This adaptability makes the instrument suitable for a wide range of applications, including clinical diagnostics, research, and teaching purposes. Blade Shape Strongly plano-concave: A plano-concave knife has one straight side and a hollow-ground side. This type of profile is extremely sharp and suitable for cutting soft, embedded materials. You'll want to avoid cutting hard materials with a plano-concave knife, which can cause the blade to vibrate. Biconcave: A biconcave knife has two hollow-ground sides. Like a plano-concave knife, a biconcave blade works best against soft materials and shouldn't be used to cut hard samples. Plano-concave: A plano-concave knife is similar to the strongly plano-concave profile except it has a thicker back. This blade can be used to cut materials that are too hard for strongly plano-concave blades, and it can also be applied to softer materials, like tissues embedded in paraffin wax. You can find plano-concave knives with various levels of concavity. Wedge-shaped: Wedge-shaped knives are more rigid than concave knives and can be used to cut harder materials. However, since wedge-shaped knives have thicker tips, they cannot be ground as sharp as concave-profile blades. Nevertheless, wedge-shaped knives can be used to cut paraffin-embedded materials, frozen sections and resin-embedded samples. Tool-edge shaped: Tool-edge knives are more stable than the other blade profiles but are also the least sharp. Tool-edge knives are commonly used to cut hard materials, synthetic resin blocks and large wax blocks. Blade Materials Steel: Disposable steel microtome blades are made of high-quality stainless steel. When held in an appropriate knife holder, disposable blades produce superior sections. You might use steel blades to cut through plant, and animal and human tissues. Glass: Glass knives can be used to slice materials for light microscopy and create very thin sections for electron microscopy. Glass knives are hard and extremely sharp, but they're also breakable, so they require careful handling. Since glass knives may deteriorate with storage, they are usually produced immediately before being used in a microtome. Diamond: Diamond microtome knives are designed to produce very thin slices for light and electron microscopy. Diamond microtome knives are incredibly durable. You can select disposable microtome blades with an amorphous diamond coating to cut through hard tissues and decalcified bone. Sapphire: Sapphire microtome knives are manufactured from artificially produced sapphire. Sapphire knives have very hard cutting edges and high durability. Sapphire knives can be used with all types of materials, but they require a particular type of knife holder. Tungsten carbide: Tungsten carbide knives are extremely hard and resistant to wear but are also brittle and must be handled carefully. Tungsten carbide knives may be used to section hard materials, such as resin-embedded tissues. Non-corrosive: Non-corrosive knives are manufactured from heat-treated stainless steel and contain 12 to 15% chromium. Non-corrosive knives are used for cryostat applications. Retraction In order to avoid damage to the specimen during the return stroke, it is necessary to move back the specimen during the return stroke so that it does not touch the blade. Vertical Stroke The vertical travel distance of the specimen holder is called the vertical stroke. A single stroke should be large enough to cover the height of the paraffin block. The standard acceptable value of the vertical stroke is 60-70mm which is long enough to cover normal paraffin blocks. Horizontal Feed The horizontal feed is the horizontal travel distance of the specimen holder. It should be long enough to cover the normal thickness of the paraffin block and is normally between 24 to 30 mm. Safety Features Manual Microtome should have some of the basic safety features like finger protection guard for disposable blades and hand-wheel lock. In addition to these a Semi-Automatic model should have feed end limit alarms. Section Thickness What section thickness is required for your tissue type. What incremental section thickness options are provided. Does it match your requirement. Section Numbers What number of sections do you plan to obtain on a day to day basis. For low throughput requirement a manual microtome would work whereas if large number of sections are required, one will need a fully automatic microtome that has a motorised sectioning. Precise Section Control If one needs to have sections with precise thickness and would prefer more ease of use, then semi-automatic microtomes should be preferred over manual models. These models use stepper motor to control feed advance compared to mechanical gears used in manual models. Moreover, if you need to move specimen quickly, bringing it closer or moving further from the blade, the same can be achieved by pressing a button on a semi-automatic microtome, whereas it requires the turning of the coarse feed wheel in manual models. Adjustable Blade Angle Make sure that the blade clearance angle can be adjusted. This is necessary to make the bevel face of the blade parallel to the cutting motion so as to obtain uniform section thickness. Adjustable specimen orientation Oftentimes one may need to re-cut sections from the same block for which it is important to precisely set and mark orientation. Neuroscience Research (Electrophysiology) Semi-Automatic Microtome are extensively used in neuroscience for preparing brain slices for electrophysiological recordings. The thick, viable sections are suitable for studying live neuronal activity. Organotypic Slice Cultures Semi-Automatic Microtome are ideal for preparing slices of organs such as the liver, kidney, or heart for organotypic culture. These cultures maintain the architecture and cell-cell interactions of the tissue, providing a more accurate representation of in vivo conditions. Immunohistochemistry For applications requiring antibody penetration into thicker tissue sections, Semi-Automatic Microtome provide the necessary thick sections that allow for better antigen-antibody interactions. Precision-Cut Tissue Slices Semi-Automatic Microtome are essential for creating precision-cut tissue slices from various organs, such as the liver , lung, and kidney. These slices are crucial for studying tissue-specific responses to drugs and other treatments in a controlled environment. The ability to maintain the tissue's structural integrity and cellular composition allows for more reliable and reproducible experiments. Histopathology Semi-Automatic Microtome are fundamental in histopathology labs for preparing thin sections of biopsies and surgical specimens (Figure 5) . These thin sections are essential for diagnosing various cancers and other pathological conditions. Pathologists rely on microtome sections to examine tissue morphology and detect abnormalities at a cellular level. Molecular Biology Thin sections are required for techniques like in situ hybridization, which allows researchers to visualize the location of specific nucleic acid sequences within the tissue. Routine Histology Semi-Automatic Microtome are used to prepare sections for routine histological staining, such as hematoxylin and eosin (H&E) staining, which provides a detailed view of the tissue architecture. Semi-Automatic Microtome Blades with the High-Precision Test The durability of microtome blades, used for sectioning paraffin blocks, was evaluated with the goal of improving the quality of sections in pathology tests. First, for the durability test of microtome blades, a sectioning test device that realizes stable sectioning operations was developed. This device comprised precise stages supported by cross-roller guides, achieving sufficient rigidness. This device allowed automated repetitive sectioning and simultaneously measured the principal and thrust cutting forces. Samples embedding porcine kidney and rib tissues were used for the durability test. Two types of blades with different blade edge angles were used. Additionally, the rake face and cross-section of blades, as well as H&E-stained sections, were observed. In the durability test with porcine kidney tissue, good quality sections were obtained even after 100 times of sectioning with both microtome blades, showing sufficient durability. However, in sectioning porcine rib tissue, the microtome blade with a large blade edge angle produced good-quality sections in the initial phase of the durability test; however, defects such as overlapping of folds were observed after 100 times of sectioning. Meanwhile, the microtome blade with a small blade edge angle experienced blade damage from the early phase of the durability test, resulting in the production of unsuitable preparations for pathology tests. These results indicated that the microtome blade with a small blade edge angle lacked durability against hard tissues such as porcine ribs. One of the most common problems encounter with semi-automatic microtome is over lubrication. Many users seem to think that if three drops of oil are called for, then six or seven or even twenty must be better. The problem with this is that oil mixed with paraffin makes a viscous gel that prevents components from tightening against one another. For example the cam in a knife holder can no longer lock against its proper bearing surface, resulting in thick/thin sections. The key to good semi-automatic microtome microtome maintenance is cleanliness and minimum lubrication. When you are done cutting for the day, remove your knife and then take the knife holder off the machine. Brush the machine off, paying particular attention to the specimen holder. After cleaning the specimen holder, check its function: Is the paddle loose? Can you twist or rotate the paddle? If so, you need to contact someone for repair. A loose paddle can slip, causing a very nasty cut! Now, engage the brake and make sure it works. With the brake on it should be significantly harder to turn the cutting handle. Check and make sure the knife holder clamp (the clamp that holds the knife holder in place) moves freely up and down. If not put two drops of oil on it and work it up and down. If it doesn't free-up, call us. Check again and make sure the knife is out and then disassemble the knife holder. When I do this I lay all the parts out, in order, in front of me. This simplifies assembly. Clean the locking cams. Remove and clean under the pressure plate. Caution! Be careful that you don't loose (as in "Ping!") the pressure plate springs! Run your fingernail across the top of the front and rear pressure plate. If your nail catches or you feel dings, replace the plate. Clean all the grooves on the knife holder base, a cotton swabbed stick simplifies this. Put 2-3 oil drops on each cam and reassemble the knife holder. On Leica machines, set the cutting angle at 4-5 degrees, and place the knife holder back on the semi-automatic microtome microtome. Trimming Trimming is normally done at a thickness of 20-30 μm. Recommend trimming the tissue to the thickness of 20-30 μm. For harder tissue or smaller tissue, it should be thinner. A standard trimming is completed when the whole tissue is exposed. Sectioning The quality microtome blade must be sharp and free of notches, and regularly replaced. This can make up for the misses in the pre-treatment process. ● Before sectioning: Tightening the knife clamper, knife holder and sample block holder, and trimming the slice until the tissue is fully exposed. ● During sectioning: The handwheel should be turned at a uniform speed. if it is too fast, it will result in uneven thickness of sectionings (slice jumping), wearing the knife holder; if it's too slow, it will thicken the sectionings. The sectioning thickness is generally set at 3~5 μm. A standard section should keep the tissue integral and uniform with an even thickness. The incomplete tissue sections may leave misdiagnosis and omission. Reminder: The microtome should be checked for proper functioning before sectioning. For hard tissues such as decalcified tissue, bone marrow, uterine fibroids and calcified tissue, the same blade edge should be used wherever possible to reduce the possibility of damage to blade. Mounting Putting the sections in the waterbath to flatten and smoothen. If the sections are still wrinkling, add alcohol solution to water as it lowers the surface tension and removes wrinkles. The high-quality slice is picked out and mounted on the slide at its 1/3 location from one of its sides. The slide will be labeled for identification. The general water temperature to float slices is about 40°C. 4 Alternate thick and thin sections It may be taken for granted that if the thickness of the section is set on the machine, the slices will come out at the desired thickness, and must be the desired thickness, otherwise the machine must be inaccurate. However, there are several other factors that can cause alternating thick and thin sections. For instance, the blade edge is not sharp or the sectioning speed is not uniform-slow sectioning results in thicker slices. Only a sharp blade with an uniform sectioning stroke can guarantee sectioning of uniform thickness. Model number KH-Q330 Brand name Kuohai Place of origin China instrument classification class I Warranty 1 year After-sale service online technical support Section thickness setting range 0.2-999um The section thickness is adjustable from 0.2 to 999 μm Specimen retraction distance 0.2μm~ section thickness is adjustable Blade Angle 0-30 degrees adjustable Paraffin clamp moving distance ≥60 mm Paraffin chuck moving distance ≥20 mm Power supply 220v,100w Weight 26kgs Size 280mmx460mmx280mm (Q: Is a microtome a semi-automatic microtome? Q: What is the most common application of semi-automatic microtome? Q: What is the benefit of semi-automatic microtome? Q: What materials are used in semi-automatic microtome? Q: What is the basic principle of semi-automatic microtome? Q: What is the daily maintenance of semi-automatic microtome? Q: How do you choose a semi-automatic microtome? Q: What is the thickness of a semi-automatic microtome? Q: Why is semi-automatic microtome important? Q: How thin can a semi-automatic microtome cut?

A semi-automated microtome combines motorized specimen feed with manual handwheel operation, providing efficient and precise sectioning for pathology and histology laboratories. Designed for medium-throughput applications, it delivers stable cutting performance and excellent section quality for paraffin tissue samples. The system is especially suitable for thick and hard tissue sectioning, ensuring consistent cutting force, smooth operation, and high precision results.