H2DCFDA (DCFH-DA)

Product Specifications

CAS Number

4091-99-0

Synonyms

2',7'-Dichlorodihydrofluorescein diacetate

Target

ROS

Applications

NF-κB

Smiles

CC (=O) OC1=CC2=C (C=C1Cl) C (C3=C (O2) C=C (OC (C) =O) C (=C3) Cl) C4=C (C=CC=C4) C (O) =O

Molecular Formula

C24H16Cl2O7

Additionnal Information

H2DCFDA (DCFH-DA) is a cell-permeable probe used to detect intracellular reactive oxygen species (ROS) production. It is de-esterified intracellularly and turns to highly fluorescent 2′,7′-dichlorofluorescein upon oxidation. It is used in sensitive and rapid quantitation of oxygen-reactive species in response to oxidative metabolism; microplate assay for detecting oxidative products in phagocytic cells, and quantitative multiwell myeloid differentiation assay. (Ex/Em=488/525 nm)