PLV3-CMV-Tet2 (mouse) -3xFLAG-CopGFP-Puro
Product Specifications
Applications
Protein Expression
Storage Temperature
37°C
Reference Sequence ID
NM_001040400.2
Aliases
Ayu17-449; E130014J05Rik; mKIAA1546
Plasmid Hos
Mammalian cell, Lentivirus
Inserted Fragment Type
ORF
Inserted Fragment Species
Mouse
Resistance
Amp
Selection antibiotic
Puro
Fluorescent labeling
Green
Competent cells
Stbl3
Nucleic Acid Sequence
AcgcgtgtagtcttatgcaatactcttgtagtcttgcaacatggtaacgatgagttagcaacatgccttacaaggagagaaaaagcaccgtgcatgccgattggtggaagtaaggtggtacgatcgtgccttattaggaaggcaacagacgggtctgacatggattggacgaaccactgaattgccgcattgcagagatattgtatttaagtgcctagctcgatacaataaacgggtctctctggttagaccagatctgagcctgggagctctctggctaactagggaacccactgcttaagcctcaataaagcttgccttgagtgcttcaagtagtgtgtgcccgtctgttgtgtgactctggtaactagagatccctcagacccttttagtcagtgtggaaaatctctagcagtggcgcccgaacagggacctgaaagcgaaagggaaaccagagctctctcgacgcaggactcggcttgctgaagcgcgcacggcaagaggcgaggggcggcgactggtgagtacgccaaaaattttgactagcggaggctagaaggagagagatgggtgcgagagcgtcagtattaagcgggggagaattagatcgcgatgggaaaaaattcggttaaggccagggggaaagaaaaaatataaattaaaacatatagtatgggcaagcagggagctagaacgattcgcagttaatcctggcctgttagaaacatcagaaggctgtagacaaatactgggacagctacaaccatcccttcagacaggatcagaagaacttagatcattatataatacagtagcaaccctctattgtgtgcatcaaaggatagagataaaagacaccaaggaagctttagacaagatagaggaagagcaaaacaaaagtaagaccaccgcacagcaagcggccactgatcttcagacctggaggaggagatatgagggacaattggagaagtgaattatataaatataaagtagtaaaaattgaaccattaggagtagcacccaccaaggcaaagagaagagtggtgcagagagaaaaaagagcagtgggaataggagctttgttccttgggttcttgggagcagcaggaagcactatgggcgcagcctcaatgacgctgacggtacaggccagacaattattgtctggtatagtgcagcagcagaacaatttgctgagggctattgaggcgcaacagcatctgttgcaactcacagtctggggcatcaagcagctccaggcaagaatcctggctgtggaaagatacctaaaggatcaacagctcctggggatttggggttgctctggaaaactcatttgcaccactgctgtgccttggaatgctagttggagtaataaatctctggaacagattggaatcacacgacctggatggagtgggacagagaaattaacaattacacaagcttaatacactccttaattgaagaatcgcaaaaccagcaagaaaagaatgaacaagaattattggaattagataaatgggcaagtttgtggaattggtttaacataacaaattggctgtggtatataaaattattcataatgatagtaggaggcttggtaggtttaagaatagtttttgctgtactttctatagtgaatagagttaggcagggatattcaccattatcgtttcagacccacctcccaaccccgaggggacccgacaggcccgaaggaatagaagaagaaggtggagagagagacagagacagatccattcgattagtgaacggatctcgacggtATCGGTtaacttttaaaagaaaaggggggattggggggtacagtgcaggggaaagaatagtagacataatagcaacagacatacaaactaaagaattacaaaaacaaattacaaaaattcaaaattttatcgatactagtattatgcccagtacatgaccttatgggactttcctacttggcagtacatctacgtattagtcatcgctattaccatggtgatgcggttttggcagtacatcaatgggcgtggatagcggtttgactcacggggatttccaagtctccaccccattgacgtcaatgggagtttgttttggcaccaaaatcaacgggactttccaaaatgtcgtaacaactccgccccattgacgcaaatgggcggtaggcgtgtacggtgggaggtTtatataagcagagctcgtttagtgaaccgtcagatcgcctggagacgccatccacgctgttttgacctccatagaagattctagagctagcCTGATACGAACTCGgaattcGCCACCatggaacaggacagaaccacccatgctgagggcaccagactgagtccattcctgatagcaccaccttctcccatcagccatacagaacctctggctgtcaaactccagaatggaagcccgttagcagagagacctcatccagaagtaaatggagacaccaagtggcaatcttcccaaagctgttatggaatatcccacatgaaaggcagccagagcagtcatgagagtccacatgaagacagagggtattccaggtgcttacagaatggagggataaaacgcacagtcagtgaaccatctctctctgggctccatccaaacaagatattgaaactagaccaaaaagctaagggagaaagcaatatcttcgaggaaagccaagaaagaaaccacggtaaaagcagccgtcagccaaatgtctccggactaagtgataatggagagcccgtgacctcgaccacccaggaaagttcaggtgcagatgctttcccaacacggaactacaatggagttgaaattcaggttctcaacgagcaggaaggggaaaaaggcaggagcgttacattacttaaaaacaagattgtgctaatgcctaatggtgctacagtttctgcccattctgaggagaacacacgtggtgaactcctggaaaaaacacagtgttatccagattgtgtctccattgccgtgcagagtaccgcatctcatgtaaacacccctagcagtcaggctgctatcgagttgtcccacgagatccctcaaccatcgcttacctcagcgcagatcaatttctcccagacctcaagcttgcagctgcctccagagccagctgcaatggtgactaaggcctgtgatgctgataatgccagtaaaccagctatagtaccaggtacctgtccttttcagaaagcagaacaccaacaaaagtcagctttggacataggcccatctcgtgcagaaaacaaaaccatccaaggaagcatggagctatttgctgaagaatactatcctagttccgaccggaatttgcaagcttcgcatggcagctctgaacagtattcaaagcaaaaggaaaccaatggtgcttacttcaggcaaagctcgaagttccctaaagattccatctctcccactactgtgaccccaccgtcacaatcacttcttgctccccgtcttgttcttcagcctcctttagaaggaaaaggcgctctaaatgatgtagctttggaagaacaccatgactaccccaaccgaagcaaccgaactcttttaagggaagggaaaatagaccatcaacccaagacatcatctagccagagtctgaatccatctgtacatacacccaacccccccttgatgcttccagaacagcatcagaatgattgtggctcaccgagccctgaaaagtcaagaaaaatgtcagaatatctcatgtattacctgccaaatcatggccacagtggaggtttacaagaacatagccaatacctgatggggcacagggagcaagagattccgaaggatgcaaacgggaaacaaacgcaaggctctgtacaggcagcacctggctggatagaactgaaagccccgaatttgcatgaagcactccatcagacaaaacgcaaggatatatccttgcactcagtcctccactctcagaccggccctgtcaatcagatgagctccaaacagtccactggcaatgtcaacatgccaggaggattccaaaggctaccttacctccagaaaacagcccagccagagcagaaggcacaaatgtaccaagtgcaagtgaaccaaggaccgtctccaggtatgggggaccaacatcttcagttccagaaagctttataccaggagtgcatccccaggacagatccgtcatctgaggctcacccgcaagcaccgagcgttcctcagtatcatttccagcaaagagtaaatccctccagtgataagcatttgagtcaacaggccacagagactcaacggttatcaggctttttacaacatactcctcagacgcaggcatcacaaacaccagcatcccagaactcaaatttccctcaaatctgccagcagcagcagcagcagcagttacagaggaagaataaagagcaaatgcctcagactttctctcatctccaaggtagcaatgataagcaaagagaaggctcgtgctttggccagattaaagtggaagaaagcttttgtgtcggaaatcagtactccaaatcaagtaatttccaaactcacaataatacccaaggggggttggagcaagtacaaaatataaataaaaattttccttattcgaagatcttaacaccaaattcgagcaacttacagattctcccttcaaatgacacacacccggcttgtgagcgggaacaagctctacatcccgtaggaagtaagacctcaaacctgcagaacatgcagtatttcccgaataatgtgaccccaaatcaggacgttcaccggtgctttcaggaacaagcgcagaagcctcagcaagcttcgtctctacaggggcttaaggacagaagccagggtgagtctccagccccaccagctgaggcagctcaacagaggtatttggtgcataatgaagcaaaggcactccctgtgcctgagcaaggaggaagtcagacacagacccctcctcagaaggacactcagaagcacgctgccttaaggtggcttctcttacagaagcaagaacagcagcaaacacagcaatcccagcctggtcataaccagatgcttaggccaatcaagactgagcctgtatccaaaccttcttcctatagataccccttgtcaccgccacaagaaaatatgtccagcaggataaagcaagagatctcctctccaagccgtgacaatgggcagccaaagagcatcattgagaccatggaacagcacctgaagcagtttcagctcaagtcactctgtgactataaggctctgactctcaagtcacagaaacacgtgaaagtgccaacagatatccaggctgcagaatcggagaaccacgcccgagctgcagagcctcaagcaaccaaaagcacagattgttctgttctcgacgatgtttcagaatcagatactcctggtgaacaaagtcagaatggcaaatgtgaaggatgcaatccagacaaagatgaagctccttattatacccatctaggagctggtcctgatgtggcagctattagaacactcatggaagaaaggtatggagagaagggtaaagctattaggattgaaaaagtcatatatactggtaaagaaggcaagagctctcagggatgtcctattgctaaatgggtatatcggagatcgagtgaggaggagaaactactgtgtttggtacgagtgcgacctaatcacacatgtgagacggcggtgatggtaattgccatcatgttgtgggacggaatcccaaagctactcgcatcagaactctactcagaacttacagatatcttgggcaagtgtggcatatgcaccaaccgtcgctgttctcagaatgaaactagaaactgttgttgtcagggtgagaatccagagacctgtggtgcctccttttcttttggttgttcttggagcatgtactataatggatgtaagtttgccagaagcaagaaaccaaggaaatttaggctacatggagctgagccaaaagaggaagagagactaggttctcatttgcaaaacctggctactgtcattgctccaatatacaagaagcttgcacccgatgcatacaataatcaggttgaatttgaacaccaagccccagactgctgtttgggtctgaaggaaggccggccattctcaggagtcactgcatgtttggacttctctgctcattcccacagagaccagcagaacatgccaaatggcagtacagtggtggtcaccctcaatagagaagacaatcgagaagtcggagctaagcctgaggatgagcagttccacgtgctgcctatgtacatcatcgcccctgaggatgagtttgggagtacggaaggccaggagaagaagatacggatggggtccattgaggttctgcagtcatttcggaggagaagggtcataaggataggagagctgcccaagagttgcaagaagaaagcggagcccaagaaagccaagaccaagaaagcagctcgaaagcgttcctctctggagaactgctccagtaggactgagaagggaaagtcttcctcacatacaaagctgatggaaaatgcaagccatatgaaacaaatgacagcacaaccgcagctttcgggcccggtcatccggcagccaccaacactccagaggcaccttcagcaagggcagaggccacagcagccgcagccacctcagccgcagccgcagacgacacctcagccacagccacagccacagcatatcatgcccggtaactctcagtctgttggttctcattgttctggatccaccagtgtctacacgagacagcctactcctcacagtccttatcccagctcagcacacacctcagatatttatggagataccaaccatgtgaacttttaccccacttcatctcatgcctcgggttcatatttgaatccttctaattacatgaacccctaccttgggcttttgaatcagaataaccaatatgcaccttttccatacaatgggagtgtgccagtggacaatggttcccctttcttaggttcttattccccccaggctcagtccagggatctacatagatatccaaaccaggaccatctcaccaatcagaacttaccacccatccacacccttcaccaacagacgtttggggacagtccctctaagtacttaagttatggaaaccaaaatatgcagagagatgccttcactactaactccaccctaaaaccaaatgtacaccacctagcaacgttttctccttaccccacccccaagatggatagtcatttcatgggagctgcctccagatcaccatacagccacccacacactgactacaaaaccagtgagcatcatctaccctctcacacgatctacagctacacggcagcagcttcggggagcagttccagccacgccttccacaacaaggagaatgacaacatagccaatgggctctcaagagtgcttccagggtttaatcatgatagaactgcttctgcccaagaactattatacagtctgactggcagcagtcaggagaagcagcctgaggtgtcaggccaggatgcagctgctgtgcaggaaattgagtattggtcagatagtgagcacaactttcaggatccttgcattggaggggtggctatagctccaactcatgggtcaattcttattgagtgtgcaaagtgtgaggttcatgccacaaccaaagtaaacgatcccgaccggaatcaccccaccaggatctcacttgtactgtataggcataagaatttgtttctaccaaaacattgtttggctctctgggaagccaaaatggctgaaaaggcccggaaagaggaagagtgcggaaagaatggatcagaccacgtgtctcagaaaaatcatggcaaacaggaaaagcgtgagcccacagggccacaggaacccagttacctgcgtttcatccagtctcttgctgagaacacagggtctgtgactacggattctaccgtgactacatcaccatatgctttcactcaggtcacagggccttacaacacatttgtaggatccGACTACAAAGACCATGACGGTGATTATAAAGATCATGACATCGACTACAAGGATGACGATGACAAGtagcggccgcGaaggatctgcgatcgctccggtgcccgtcagtgggcagagcgcacatcgcccacagtccccgagaagttggggggaggggtcggcaattgaacgggtgcctagagaaggtggcgcggggtaaactgggaaagtgatgtcgtgtactggctccgcctttttcccgagggtgggggagaaccgtatataagtgcagtagtcgccgtgaacgttctttttcgcaacgggtttgccgccagaacacagctgaagcttcgaggggctcgcatctctccttcacgcgcccgccgccctacctgaggccgccatccacgccggttgagtcgcgttctgccgcctcccgcctgtggtgcctcctgaactgcgtccgccgtctaggtaagtttaaagctcaggtcgagaccgggcctttgtccggcgctcccttggagcctacctagactcagccggctctccacgctttgcctgaccctgcttgctcaactctacgtctttgtttcgttttctgttctgcgccgttacagatccaagctgtgaccggcgcctacgctagacgccaccatggagagcgacgagagcggcctgcccgccatggagatcgagtgccgcatcaccggcaccctgaacggcgtggagttcgagctggtgggcggcggagagggcacccccaagcagggccgcatgaccaacaagatgaagagcaccaaaggcgccctgaccttcagcccctacctgctgagccacgtgatgggctacggcttctaccacttcggcacctaccccagcggctacgagaaccccttcctgcacgccatcaacaacggcggctacaccaacacccgcatcgagaagtacgaggacggcggcgtgctgcacgtgagcttcagctaccgctacgaggccggccgcgtgatcggcgacttcaaggtggtgggcaccggcttccccgaggacagcgtgatcttcaccgacaagatcatccgcagcaacgccaccgtggagcacctgcaccccatgggcgataacgtgctggtgggcagcttcgcccgcaccttcagcctgcgcgacggcggctactacagcttcgtggtggacagccacatgcacttcaagagcgccatccaccccagcatcctgcagaacgggggccccatgttcgccttccgccgcgtggaggagctgcacagcaacaccgagctgggcatcgtggagtaccagcacgccttcaagacccccatcgccttcgccagatcccgcgctcagtcgtccaattctgccgtggacggcaccgccggacccggctccaccggatctcgcgagggcagaggaagtcttctaacatgcggtgacgtggaggagaatcccggccctatgaccgagtacaagcccacggtgcgcctcgccacccgcgacgacgtccccagggccgtacgcaccctcgccgccgcgttcgccgactaccccgccacgcgccacaccgtcgatccggaccgccacatcgagcgggtcaccgagctgcaagaactcttcctcacgcgcgtcgggctcgacatcggcaaggtgtgggtcgcggacgacggcgccgcggtggcggtctggaccacgccggagagcgtcgaagcgggggcggtgttcgccgagatcggcccgcgcatggccgagttgagcggttcccggctggccgcgcagcaacagatggaaggcctcctggcgccgcaccggcccaaggagcccgcgtggttcctggccaccgtcggcgtctcgcccgaccaccagggcaagggtctgggcagcgccgtcgtgctccccggagtggaggcggccgagcgcgccggggtgcccgccttcctggagacctccgcgccccgcaacctccccttctacgagcggctcggcttcaccgtcaccgccgacgtcgaggtgcccgaaggaccgcgcacctggtgcatgacccgcaagcccggtgcctgaaatcaacctctggattacaaaatttgtgaaagattgactggtattcttaactatgttgctccttttacgctatgtggatacgctgctttaatgcctttgtatcatgctattgcttcccgtatggctttcattttctcctccttgtataaatcctggttgctgtctctttatgaggagttgtggcccgttgtcaggcaacgtggcgtggtgtgcactgtgtttgctgacgcaacccccactggttggggcattgccaccacctgtcagctcctttccgggactttcgctttccccctccctattgccacggcggaactcatcgccgcctgccttgcccgctgctggacaggggctcggctgttgggcactgacaattccgtggtgttgtcggggaaATCATCGTCCTTTCCTtggctgctcgcctgtgttgccacctggattctgcgcgggacgtccttctgctacgtcccttcggccctcaatccagcggaccttccttcccgcggcctgctgccggctctgcggcctcttccgcgtctTcgccttcgccctcagacgagtcggatctccctttggGccgcctccccgcctggtacctttaagaccaatgacttacaaggcagctgtagatcttagccactttttaaaagaaaaggggggactggaagggctaattcactcccaacgaaGataagatctgctttttgcttgtactgggtctctctggttagaccagatctgagcctgggagctctctggctaactagggaacccactgcttaagcctcaataaagcttgccttgagtgcttcaagtagtgtgtgcccgtctgttgtgtgactctggtaactagagatccctcagacccttttagtcagtgtggaaaatctctagcagtagtagttcatgtcatcttattattcagtatttataacttgcaaagaaatgaatatcagagagtgagaggaacttgtttattgcagcttataatggttacaaataaagcaatagcatcacaaatttcacaaataaagcatttttttcactgcattctagttgtggtttgtccaaactcatcaatgtatcttatcatgtctggctctagctaTCCCGCCCCTAACTCCGCCCAtcccgcccctaactccgcccagttccgcccattctccgccccatggctgactaattttttttatttatgcagaggccgaggccgcctcggcctctgagctattccagaagtagtgaggaggcttttttggaggcctagacttttgcagagaccaaattcgtaatcatgtcatagctgtttcctgtgtgaaattgttatccgctcacaattccacacaacatacgagccggaagcataaagtgtaaagcctggggtgcctaatgagtgagctaactcacattaattgcgttgcgctcactgcccgctttccagtcgggaaacctgtcgtgccagctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgctcttccgcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccataggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaaggacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagttaccaatgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagtgctgcaatgataccgcgagacccacgctcaccggctccagatttatcagcaataaaccagccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctattaattgttgccgggaagctagagtaagtagttcgccagttaatagtttgcgcaacgttgttgccattgctacaggcatcgtggtgtcacgctcgtcgtttggtatggcttcattcagctccggttcccaacgatcaaggcgagttacatgatcccccatgttgtgcaaaaaagcggttagctccttcggtcctccgatcgttgtcagaagtaagttggccgcagtgttatcactcatggttatggcagcactgcataattctcttactgtcatgccatccgtaagatgcttttctgtgactggtgagtactcaaccaagtcattctgagaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaatacgggataataccgcgccacatagcagaactttaaaagtgctcatcattggaaaacgttcttcggggcgaaaactctcaaggatcttaccgctgttgagatccagttcgatgtaacccactcgtgcacccaactgatcttcagcatcttttactttcaccagcgtttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcgacacggaaatgttgaatactcatactcttcctttttcaatattattgaagcatttatcagggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggggttccgcgcacatttccccgaaaagtgccacctgacgtctaagaaaccattattatcatgacattaacctataaaaataggcgtatcacgaggccctttcgtctcgcgcgtttcggtgatgacggtgaaaacctctgacacatgcagctcccggagacggtcacagcttgtctgtaagcggatgccgggagcagacaagcccgtcagggcgcgtcagcgggtgttggcgggtgtcggggctggcttaactatgcggcatcagagcagattgtactgagagtgcaccatatgcggtgtgaaataccgcacagatgcgtaaggagaaaataccgcatcaggcgccattcgccattcaggctgcgcaactgttgggaagggcgatcggtgcgggcctcttcgctattacgccagctggcgaaagggggatgtgctgcaaggcgattaagttgggtaacgccagggttttcccagtcacgacgttgtaaaacgacggccagtgccaagctg
Gene Length
14026
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