PMLLV-CMV-EPRS (mouse) -3xFLAG-EF1a-CopGFP-T2A-Puro
Product Specifications
Applications
Protein Expression
Storage Temperature
37°C
Reference Sequence ID
NM_001357474.1
Plasmid Hos
Mammalian cell, Lentivirus
Inserted Fragment Type
ORF
Inserted Fragment Species
Mouse
Resistance
Amp
Selection antibiotic
Puro
Fluorescent labeling
Green
Competent cells
Stbl3
Nucleic Acid Sequence
AcgcgtgtagtcttatgcaatactcttgtagtcttgcaacatggtaacgatgagttagcaacatgccttacaaggagagaaaaagcaccgtgcatgccgattggtggaagtaaggtggtacgatcgtgccttattaggaaggcaacagacgggtctgacatggattggacgaaccactgaattgccgcattgcagagatattgtatttaagtgcctagctcgatacaataaacgggtctctctggttagaccagatctgagcctgggagctctctggctaactagggaacccactgcttaagcctcaataaagcttgccttgagtgcttcaagtagtgtgtgcccgtctgttgtgtgactctggtaactagagatccctcagacccttttagtcagtgtggaaaatctctagcagtggcgcccgaacagggacctgaaagcgaaagggaaaccagagctctctcgacgcaggactcggcttgctgaagcgcgcacggcaagaggcgaggggcggcgactggtgagtacgccaaaaattttgactagcggaggctagaaggagagagatgggtgcgagagcgtcagtattaagcgggggagaattagatcgcgatgggaaaaaattcggttaaggccagggggaaagaaaaaatataaattaaaacatatagtatgggcaagcagggagctagaacgattcgcagttaatcctggcctgttagaaacatcagaaggctgtagacaaatactgggacagctacaaccatcccttcagacaggatcagaagaacttagatcattatataatacagtagcaaccctctattgtgtgcatcaaaggatagagataaaagacaccaaggaagctttagacaagatagaggaagagcaaaacaaaagtaagaccaccgcacagcaagcggccactgatcttcagacctggaggaggagatatgagggacaattggagaagtgaattatataaatataaagtagtaaaaattgaaccattaggagtagcacccaccaaggcaaagagaagagtggtgcagagagaaaaaagagcagtgggaataggagctttgttccttgggttcttgggagcagcaggaagcactatgggcgcagcctcaatgacgctgacggtacaggccagacaattattgtctggtatagtgcagcagcagaacaatttgctgagggctattgaggcgcaacagcatctgttgcaactcacagtctggggcatcaagcagctccaggcaagaatcctggctgtggaaagatacctaaaggatcaacagctcctggggatttggggttgctctggaaaactcatttgcaccactgctgtgccttggaatgctagttggagtaataaatctctggaacagattggaatcacacgacctggatggagtgggacagagaaattaacaattacacaagcttaatacactccttaattgaagaatcgcaaaaccagcaagaaaagaatgaacaagaattattggaattagataaatgggcaagtttgtggaattggtttaacataacaaattggctgtggtatataaaattattcataatgatagtaggaggcttggtaggtttaagaatagtttttgctgtactttctatagtgaatagagttaggcagggatattcaccattatcgtttcagacccacctcccaaccccgaggggacccgacaggcccgaaggaatagaagaagaaggtggagagagagacagagacagatccattcgattagtgaacggatctcgacggttaacttttaaaagaaaaggggggattggggggtacagtgcaggggaaagaatagtagacataatagcaacagacatacaaactaaagaattacaaaaacaaattacaaaaattcaaaattttatcgatactagtattatgcccagtacatgaccttatgggactttcctacttggcagtacatctacgtattagtcatcgctattaccatggtgatgcggttttggcagtacatcaatgggcgtggatagcggtttgactcacggggatttccaagtctccaccccattgacgtcaatgggagtttgttttggcaccaaaatcaacgggactttccaaaatgtcgtaacaactccgccccattgacgcaaatgggcggtaggcgtgtacggtgggaggtctatataagcagagctcgtttagtgaaccgtcagatcgcctggagacgccatccacgctgttttgacctccatagaagattctagagctagccaccatggcggcgctgtgcctcacggtgaacgccggaaaccctccgctggaagctctgctggcggtggaacatgtgaaaggtgatgtcagcatttctgtggaagaagggaaagagaatcttcttcgggtttctgagactgtggcattcactgacgtgaattcaatcctgcgctacctggctagaattgcaactacgtctgggctgtatgggactaacctgatggagcacactgagattgatcattggttggagtttagtgctacaaagttgtcttcgtgtgataggctgacttctgcaatcaacgagcttaatcattgcctgtctctgagaacatacctagttgggaactccttgaccttagcagatctatgtgtttgggccaccctaaaaggaagtgctgcatggcaggaacacttgaaacagaacaaaactctagtccacgttaaacgctggtttggctttcttgaggcccagcaggccttccgctcagttggcaccaagtgggatgtttcaggaaacagagctacagtggcacctgacaaaaaacaagatgttgggaaattcgtggagcttccaggtgcagagatgggaaaggtgacagtcagattccccccagaggctagtggttacttacacattgggcatgcgaaagctgctcttctgaaccagcactaccaggttaactttaaagggaaactgatcatgagatttgatgacacaaacccagaaaaggaaaaggaagattttgagaaggtcatcttggaagatgttgcaatgctgcatattaaaccagatcagtttacttacacttcagatcattttgaaactataatgaagtatgcagagaaattaattcaagaagggaaggcttatgtggatgacactcctgcagaacagatgaaggcggaacgtgaacagaggacagaatccaaacacaggaagaactctgttgagaagaatctacagatgtgggaagaaatgaaaaaaggaagccagtttggtcagtcctgttgcctgcgagcaaaaattgacatgagtagtaacaatgggtgtatgagagacccgaccctttaccgatgcaaaatccagccccacccaaggactgggaataaatacaatgtttatccaacatatgactttgcctgccctatagtggacagcattgaaggtgttactcatgccttgaggacaacagagtaccacgaccgagatgaacaattttattggatcattgaagctttaggcataaggaaaccatacatctgggagtacagtcgcttaaatctcaacaacaccgtgctgtccaaaagaaagctcacgtggtttgtcaatgaaggactggtagatggatgggatgacccacggtttcctacagttcgtggagttctgagaagagggatgacagttgaggggctgaagcagttcattgctgctcagggctcctcaaggtctgttgtgaacatggaatgggacaaaatctgggcgttcaacaaaaagctgcgagctctctgtaagaaggttattgacccagtggctccacggtacgttgcattgctgaagaaggaagtcgtcccagtgaatgtcctggatgcgcaggaggagatgaaggaagtagccagacaccccaagaatcctgatgttggtttgaagcctgtgtggtacagtcccaaggttttcatagaaggggccgatgcagagactttctcagagggtgagatggtcacatttataaactggggcaacatcaacattactaaaatacacaaaaatgcagatggaaaaattacatctctagatgcaaaattgaatttggaaaacaaagactacaagaaaaccaccaagatcacttggcttgctgagagcacacacgctctctcgatcccagccgtctgtgtcacttatgagcacttgatcacaaagccagtgctgggaaaagatgaggacttcaagcagtacatcaacaaagacagtaagcatgaagagctaatgctaggagatccttgccttaaagatctgaaaaaaggagacatcatacaactgcagaggagagggttcttcatatgcgaccagccatacgaacctgtcagcccatatagttgcagagaagctccatgcatcctgatatacatacctgatgggcacacaaaggaaatgccaacatccggatcaaaggaaaagaccaaagtggaaatctcaaaaaaggagacaagctctgctcctaaagaaagaccagcacctgctgtgagttctacttgtgctacagctgaggattcctccgtcctctacagcagagtggcggtgcagggggatgtggttcgtgaattgaaagccaagaaagcgccaaaggaagatatagacgcagctgtgaagcagcttttgactttgaaagctgaatataaagagaaaactgggcaggagtataaacctgggaacccctctgctgcagcagtacagactgtttctacaaagtcatcatcaaacactgtggaaagtacatctttatacaataaagtcgctgcccaaggggaggtggtccgaaagctgaaagcggaaaaggctcctaaggctaaagtaactgaagctgtggaatgtttgctgtccctgaaagctgagtataaagaaaagactgggaaggactatgtacctggccagcccccagcatcgcagaactcacattcaaaccctgtcagtaatgctcagcctgctggagcggagaaaccagaagccaaagtgctatttgacagagtggcttgtcaaggagaagtagttcgaaaacttaaagctgaaaaggcctctaaggatcaagtggattcggcggtacaagaacttcttcagctgaaggcacagtacaagtcgctgacaggaatcgagtataagcctgtgtctgctactggggcagaagacaaagataaaaagaagaaagaaaaagaaaataaatctgaaaagcagaacaagcctcagaaacaaaatgatggccaagggaaagactcttccaaaagccaaggcagtggcctgtcatcaggtggagcaggagaagggcaggggcctaagaaacagaccagattgggtcttgaagcaaaaaaagaagaaaatcttgccgaatggtactctcaagtcatcacaaagtccgagatgattgaatactacgatgtgagtggctgctatatcctccgtccctggtcgtactccatctgggagtccattaaggacttctttgacgctgagatcaagaagctcggtgttgagaactgctacttccccatatttgtgtctcaggctgcgttagagaaagagaagaatcacatcgaagactttgctcctgaggttgcttgggttacaagatcaggaaaaacagagttggcagagccaattgccattcgtcctactagtgaaacagtgatgtatccggcatatgctaaatgggtgcagtcccacagagacctgcccgtcaggctcaaccagtggtgcaatgtggtgcgctgggagttcaaacacccacagcctttcctccgaactcgggagttcctgtggcaggaggggcacagcgcctttgccacctttgaggaagcagctgatgaggttttacagatccttgagttatatgctcgagtatatgaagagctcttagccattcctgttgtgagaggaaggaagactgaaaaggagaagtttgcaggaggagactacacaaccacaatagaagccttcatatctgccagcggacgcgccattcagggagcaacatcacaccatttaggccagaatttctccaaaatgtgtgaaatagtttttgaagatcccaagacaccaggagaaaagcagtttgcataccagtgctcctggggcctgacaacacgaaccatcggtgtgatggtcatggtccatggggacaacatgggcctagtgcttccaccccgagtagcatctgtccaggtggtagtcattccctgtggaatcacaaatgccctgtctgaagaagacagagaggcactgatggcaaaatgcaacgagtacagaaggcggctgcttggtgctaacatccgagtccgagtcgatctacgagacaactactccccaggctggaagttcaaccactgggaactcaagggagttccagtcagacttgaggtggggccacgtgatatgaagagctgtcagtttgtagcagtcagacgagatactggagagaaactgacaattgctgagaaggaggctgaggctaagctggaaaaggttttggaagacatccagctgaaccttttcacaagggcttctgaagaccttaaaacccacatggttgtgtctaatacactggaggacttccagaaggtgctagatgctggaaaggttgcacagattccattttgtggggaaattgactgtgaggactggatcaaaaagatgactgccagggatcaagatgtggaacctggtgctccatccatgggagccaaaagcctttgcattcctttcaaccctctgtgtgagctgcagcccggagccatgtgtgtctgtggcaagaatcctgccaagttctacaccttgtttggtcggagttacggatccGACTACAAAGACCATGACGGTGATTATAAAGATCATGACATCGACTACAAGGATGACGATGACAAGTGAgcggccgcGaaggatctgcgatcgctccggtgcccgtcagtgggcagagcgcacatcgcccacagtccccgagaagttggggggaggggtcggcaattgaacgggtgcctagagaaggtggcgcggggtaaactgggaaagtgatgtcgtgtactggctccgcctttttcccgagggtgggggagaaccgtatataagtgcagtagtcgccgtgaacgttctttttcgcaacgggtttgccgccagaacacagctgaagcttcgaggggctcgcatctctccttcacgcgcccgccgccctacctgaggccgccatccacgccggttgagtcgcgttctgccgcctcccgcctgtggtgcctcctgaactgcgtccgccgtctaggtaagtttaaagctcaggtcgagaccgggcctttgtccggcgctcccttggagcctacctagactcagccggctctccacgctttgcctgaccctgcttgctcaactctacgtctttgtttcgttttctgttctgcgccgttacagatccaagctgtgaccggcgcctacgctagacgccaccatggagagcgacgagagcggcctgcccgccatggagatcgagtgccgcatcaccggcaccctgaacggcgtggagttcgagctggtgggcggcggagagggcacccccaagcagggccgcatgaccaacaagatgaagagcaccaaaggcgccctgaccttcagcccctacctgctgagccacgtgatgggctacggcttctaccacttcggcacctaccccagcggctacgagaaccccttcctgcacgccatcaacaacggcggctacaccaacacccgcatcgagaagtacgaggacggcggcgtgctgcacgtgagcttcagctaccgctacgaggccggccgcgtgatcggcgacttcaaggtggtgggcaccggcttccccgaggacagcgtgatcttcaccgacaagatcatccgcagcaacgccaccgtggagcacctgcaccccatgggcgataacgtgctggtgggcagcttcgcccgcaccttcagcctgcgcgacggcggctactacagcttcgtggtggacagccacatgcacttcaagagcgccatccaccccagcatcctgcagaacgggggccccatgttcgccttccgccgcgtggaggagctgcacagcaacaccgagctgggcatcgtggagtaccagcacgccttcaagacccccatcgccttcgccagatcccgcgctcagtcgtccaattctgccgtggacggcaccgccggacccggctccaccggatctcgcgagggcagaggaagtcttctaacatgcggtgacgtggaggagaatcccggccctatgaccgagtacaagcccacggtgcgcctcgccacccgcgacgacgtccccagggccgtacgcaccctcgccgccgcgttcgccgactaccccgccacgcgccacaccgtcgatccggaccgccacatcgagcgggtcaccgagctgcaagaactcttcctcacgcgcgtcgggctcgacatcggcaaggtgtgggtcgcggacgacggcgccgcggtggcggtctggaccacgccggagagcgtcgaagcgggggcggtgttcgccgagatcggcccgcgcatggccgagttgagcggttcccggctggccgcgcagcaacagatggaaggcctcctggcgccgcaccggcccaaggagcccgcgtggttcctggccaccgtcggcgtctcgcccgaccaccagggcaagggtctgggcagcgccgtcgtgctccccggagtggaggcggccgagcgcgccggggtgcccgccttcctggagacctccgcgccccgcaacctccccttctacgagcggctcggcttcaccgtcaccgccgacgtcgaggtgcccgaaggaccgcgcacctggtgcatgacccgcaagcccggtgcctgaaatcaacctctggattacaaaatttgtgaaagattgactggtattcttaactatgttgctccttttacgctatgtggatacgctgctttaatgcctttgtatcatgctattgcttcccgtatggctttcattttctcctccttgtataaatcctggttgctgtctctttatgaggagttgtggcccgttgtcaggcaacgtggcgtggtgtgcactgtgtttgctgacgcaacccccactggttggggcattgccaccacctgtcagctcctttccgggactttcgctttccccctccctattgccacggcggaactcatcgccgcctgccttgcccgctgctggacaggggctcggctgttgggcactgacaattccgtggtgttgtcggggaagctgacgtcctttccatggctgctcgcctgtgttgccacctggattctgcgcgggacgtccttctgctacgtcccttcggccctcaatccagcggaccttccttcccgcggcctgctgccggctctgcggcctcttccgcgtctccgccttcgccctcagacgagtcggatctccctttggccgcctccccgcctggtacctttaagaccaatgacttacaaggcagctgtagatcttagccactttttaaaagaaaaggggggactggaagggctaattcactcccaacgaaaataagatctgctttttgcttgtactgggtctctctggttagaccagatctgagcctgggagctctctggctaactagggaacccactgcttaagcctcaataaagcttgccttgagtgcttcaagtagtgtgtgcccgtctgttgtgtgactctggtaactagagatccctcagacccttttagtcagtgtggaaaatctctagcagtagtagttcatgtcatcttattattcagtatttataacttgcaaagaaatgaatatcagagagtgagaggaacttgtttattgcagcttataatggttacaaataaagcaatagcatcacaaatttcacaaataaagcatttttttcactgcattctagttgtggtttgtccaaactcatcaatgtatcttatcatgtctggctctagctatcccgcccctaactccgcccagttccgcccattctccgccccatggctgactaattttttttatttatgcagaggccgaggccgcctcggcctctgagctattccagaagtagtgaggaggcttttttggaggcctagacttttgcagagacggcccaaattcgtaatcatggtcatagctgtttcctgtgtgaaattgttatccgctcacaattccacacaacatacgagccggaagcataaagtgtaaagcctggggtgcctaatgagtgagctaactcacattaattgcgttgcgctcactgcccgctttccagtcgggaaacctgtcgtgccagctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgctcttccgcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccataggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaaggacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagttaccaatgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagtgctgcaatgataccgcgagacccacgctcaccggctccagatttatcagcaataaaccagccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctattaattgttgccgggaagctagagtaagtagttcgccagttaatagtttgcgcaacgttgttgccattgctacaggcatcgtggtgtcacgctcgtcgtttggtatggcttcattcagctccggttcccaacgatcaaggcgagttacatgatcccccatgttgtgcaaaaaagcggttagctccttcggtcctccgatcgttgtcagaagtaagttggccgcagtgttatcactcatggttatggcagcactgcataattctcttactgtcatgccatccgtaagatgcttttctgtgactggtgagtactcaaccaagtcattctgagaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaatacgggataataccgcgccacatagcagaactttaaaagtgctcatcattggaaaacgttcttcggggcgaaaactctcaaggatcttaccgctgttgagatccagttcgatgtaacccactcgtgcacccaactgatcttcagcatcttttactttcaccagcgtttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcgacacggaaatgttgaatactcatactcttcctttttcaatattattgaagcatttatcagggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggggttccgcgcacatttccccgaaaagtgccacctgacgtctaagaaaccattattatcatgacattaacctataaaaataggcgtatcacgaggccctttcgtctcgcgcgtttcggtgatgacggtgaaaacctctgacacatgcagctcccggagacggtcacagcttgtctgtaagcggatgccgggagcagacaagcccgtcagggcgcgtcagcgggtgttggcgggtgtcggggctggcttaactatgcggcatcagagcagattgtactgagagtgcaccatatgcggtgtgaaataccgcacagatgcgtaaggagaaaataccgcatcaggcgccattcgccattcaggctgcgcaactgttgggaagggcgatcggtgcgggcctcttcgctattacgccagctggcgaaagggggatgtgctgcaaggcgattaagttgggtaacgccagggttttcccagtcacgacgttgtaaaacgacggccagtgccaagctg
Gene Length
12803
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