PCMV-SI (pig) -3xFLAG-Neo
Product Specifications
Applications
Protein Expression
Storage Temperature
37°C
Plasmid Hos
Mammalian cell
Inserted Fragment Type
ORF
Inserted Fragment Species
Pig
Resistance
Amp
Selection antibiotic
Neo/G418
Competent cells
DH5a
Nucleic Acid Sequence
GTTAGGCGTTTTGCGCTGCTTCGCGATGTACGGGCCAGATATACGCGTTGACATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGGGTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCCGCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCATAGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGCCCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTACGCCCCCTATTGACGTCAATGACGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTATGGGACTTTCCTACTTGGCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACATCAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGTCAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAACAACTCCGCCCCATTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCTCTGGCTAACTAGAGAACCCACTGCTTACTGGCTTATCGAAATTAATACGACTCACTATAGGGAGACCCAAGCTGGCTAGCGTTTAAACTTAAGCTTGGTACCGAGCTCGAATTCGCCACCatggcaaggaagaaattttctggattagaaatctctctgatcgtcctctttgctatagttacaataatagctattgccctagtggttgttttagcaactaagacacctgctgttgaagaaattagtgattctacttcaaccccagctaccactcgtacaaccactccatatcctggttctggaaaatgtccaagtgaactaaacgatcctatcaatgagagaataaactgcattccagatcaatttccaacacagacactttgtgcaactcgaggatgctgctggaagccatggaatgattctattattccttggtgcttctttgtagataatcatggctataatgttgagggaacaacatcaacaaatactggacttgaagccaaattaaacaggataccatcacctacattatttggagatgatattaacagtgttctcctcacaacccaaagtcagacacccaatcgtttccggttcaagattactgacccaaataataggagatatgaagttcctcatcaatttgtaaaagaatttagtggaacagcagcttctgatacattgtatgatgtgcaggttatagaaaatccatttagcatcaaaattattagaaaaagcaataatagaattttgtttgacaccagcattggtccactggtatactctgaccagtatttacagctctctgccagactttccagtgaatatatttatgggattggggaacatattcataagagattccgccatgacttatactggaaaacatggccaatttttactcgggatcaacttcctggtgataataataataatttatatggccatcaaacattcttcatgtgcattgaagatacttctggaaagtcatttggtgttttcctaatgaatagcaatgcaatggagatttttatccagcctactccaatagtaacttacagaattataggtggcattctggacttttacatctttctaggagatacaccagaacaagtagttcagcagtatcaagagctcattgggcggccagcaatgccagcatattggagtcttggattccaactgagtcgctgggattataaatcacttgatgtagtgaaagaagtggtacagagaaatcgggaagctggcataccatttgatacacaggtcactgatattgactatatggaagacaaaaaagactttacttatgatcacgttgcatttaatgggctccctgaatttgttcaagatttgcatgaccacggtcagaaatatgtcatcatcttggaccctgcaatttccatacagaaacttgccagtggagcagcatatgaaacctatgataggggaactgcacaaaatgtttggataaatgagtcagatggaactacagcaattattggagaggtatggccaggattaacagtataccctgatttcactagtccgaactgcatagaatggtgggcaaatgagtgcagcattttccatcaagtagtgaattatgatgggctttggattgacatgaacgaagtttccagctttgttcatggctcagagaaaggatgcagtgacaacaaactaaattatcctccttttactccaggtattcttgacaaactcctgtatgccaaaacagtttgcatggattctgtgcagtactgggggaaacagtacgatgttcatagcctctatggatacagcatggctatagctacagagaaagctgtagaaaaagtttttcgtaataaaagaagctttattctcacccggtcaacttttgctggatctggaagtcatgctgcacattggttaggagacaatactgcttcatgggaacaaatggaatggtctattacaggaatgctggagttcagtttgtttggaatacctttggttggggcagatatctgtggatttgtggttgaaaccacagaagaactttgccgaaggtggatgcaacttggggctttttatccattttccagaaaccataatgcccagggatatgagcatcaggatccagcattttttggacagaattctcttctggttaattcatcaaagcactatttgaatattcgttataccttgttgcctttcctctacactttattttataaagcccatatgtttggagaaacagttgtaaggccaattcttcatgagttttatgaggatactaatagctggattgaagacactcagttcttatggggcccctcattacttattacccctgtcttgaaacagggagcaagtacagtgagtgcatatatccctaatgctacttggtatgactatgaaactggtgcaaaaaggccatggagaaaacaacgtgttgatatgtatcttccagcagataaaataggattacatcttagaggggggtatattattcccatccaacaacctgctgtaactacaactgccagccgaaagaatcctctaggccttatagttgcattagatgaatataacacagcagaaggagactttttctgggatgatggagaaactaaagataccatacaaaatggcagctacattttctatacattttcagtttctaataacaaattagatattatgtgcacacattcatcataccaagaaggaaccactttagcatttgagactataaaaatccttgggttgatagacgcggttacagaagtttcagtggtggaaggtgatcaaccaatgagtgctcactacaatttcacttatgatgcttccaaccagtatctactaatttacaatctcaagtttaaccttggaagaaactttactgttcaatgggatcaaactttcttggacgctgaaaagtttacttgttatccagatacagacacaataactaaagaactctgtgaagaacgtggctgcttatggcaagagtctcatttggaatccaaagcacctgagtgtttctttcctaaagaagacaactcttatttagtccagtcaactcagtactcatcactgggtgtaacagctgacctccagctgaatactgcaagtacccgaataaagctaccttctgatcccatcccaactcttcgtgtggaggtgaaatatcacaaagattatatgctgcagtttaagatttatgatccccaaaataagagatatgaagttccagtaccattaaacattccaactacaccaacaagtacttatgaaaataggctttatgatgttgaaatcaaggaaaatccttttggcatccagattcgaagaagaagcacaggaaaagttatctgggattctcgcctacctggatttgcttttaacaaccagttcattcaaatatctacacgtctgccatcagaatatatatatggttttggagaagtagaacacacggcatttaagcgagatctgaactggaatacttggggaatgttcaccagagaccaaccccccggttataaacttaattcttatggatttcatccctattacatggctctggaggatgagagccatgctcatggagttctcctacttaacagcaatgcaatggatgttacatttcagccaactcctgctctaacatatcgcataattggaggaatcttggacttttatatgtttttgggcccatctccagaagttgcaacaaagcaataccatgaagtaattggccgaccagtcatgccaccttattgggctttgggattccagttgtgtcgttatgggtacagaaatacttcacaagttgaagaagtgtataatgacatggtgactgctcagatcccctatgatgttcaatacacagacattgattacatggaaaggcagctagactttacgattggtgaagaattccgtgaacttcctaagtttgttgacaaaataagaaatgagggaatgagatacattattatcctggatccggcaatttcaggaaatgaaacagagccttaccctgcctttgatagaggacaggagaaagatgtttttgtcaaatggcctaacaccagtgatatttgttgggcaaaggtttggccagatttgcccaatgtaacaatagatgaaagtctaactgaagatgaagctgttaatgcctccagagctcatgtagcttttccagatttctttaggaattccacagcagaatggtgggcaagagaaattatagatttctacaataaccagatgaagtttgatggcttgtggattgacatgaatgagccatcaagttttgtaaacggaacaactactaatcaatgcagaaatacagagctaaattatccaccttattttccagaactcacaaaaagaactgaaggattacatttcagaaccatgtgtatggaaaccgagcagattcttagtgatggatcatctgttttgcattatgacgttcacaatctatatggatggtcacaagtgaaacctacttatgatgcattgcagaaggcaactggcaaaagaggaattgttatttctcggtccacatatcctactgctggacgttgggcaggacactggcttggagacaactatgcaaaatgggacaacttggacaaatctatcattggtatgatggaattcagtctctttggaatatcatatactggagcagatatctgtggcttcttcaacaattcggaatatcagctctgtgcccgctggatgcaacttggagcattctatccatattcaagaaatcacaacattgcatttactagaagacaagaccctgcctcctggaatgaaactttttctgaaatgtcaaggaatattctaaatattagatacactttattgccctatttctatacacagatgcatgaaatccatgcttatggtggcactgttatccgaccccttttgcatgagttctttaatgaaaagccaacctgggatatattcaaacagttcctatggggtccagcctttttggttactcctgtattggaaccttttgctgacactgtacaaggctatgtccctaatgctcggtggtttgattaccatacaggcaaggatattggtatcagagaaacatttcacatattttcagctcctttatacgaaataaacctacatgtccgtggtggtcatattctaccatgtcaagagcctgctcaaaacacatttttcagtcgacaaaatttcatgaagctcattgttgctgcagatgataatcagacggcacaaggaaatctgttttgggatgatggagagacaattgacacctatgaaagagacttatatttcttggtacaatttaattttaacaagtccatcttgacaagcactatactgaaaaatagttacataaacagaaatgaaatgaggcttggatatgtcctcatatgggggaaagaaaagacttctgttaatgaagttaatctcatatataatggaaataaagagactgttccctttgttgaagacctaaaccaggagatattaaatattgatctaagagggttaaatgttactctagatgaaccaatagaaatcagctggtcaGGATCCGACTACAAAGACCATGACGGTGATTATAAAGATCATGACATCGACTACAAGGATGACGATGACAAGTAGTGAGGGCCCGATATCTCGACAATCAACCTCTGGATTACAAAATTTGTGAAAGATTGACTGGTATTCTTAACTATGTTGCTCCTTTTACGCTATGTGGATACGCTGCTTTAATGCCTTTGTATCATGCTATTGCTTCCCGTATGGCTTTCATTTTCTCCTCCTTGTATAAATCCTGGTTGCTGTCTCTTTATGAGGAGTTGTGGCCCGTTGTCAGGCAACGTGGCGTGGTGTGCACTGTGTTTGCTGACGCAACCCCCACTGGTTGGGGCATTGCCACCACCTGTCAGCTCCTTTCCGGGACTTTCGCTTTCCCCCTCCCTATTGCCACGGCGGAACTCATCGCCGCCTGCCTTGCCCGCTGCTGGACAGGGGCTCGGCTGTTGGGCACTGACAATTCCGTGGTGTTGTCGGGGAAGCTGACGTCCTTTCCATGGCTGCTCGCCTGTGTTGCCACCTGGATTCTGCGCGGGACGTCCTTCTGCTACGTCCCTTCGGCCCTCAATCCAGCGGACCTTCCTTCCCGCGGCCTGCTGCCGGCTCTGCGGCCTCTTCCGCGTCTTCGCCTTCGCCCTCAGACGAGTCGGATCTCCCTTTGGGCCGCCTCCCCGCCTGGAAACGGGGGAGGCTAACTGAAACACGGAAGGAGACAATACCGGAAGGAACCCGCGCTATGACGGCAATAAAAAGACAGAATAAAACGCACGGGTGTTGGGTCGTTTGTTCATAAACGCGGGGTTCGGTCCCAGGGCTGGCACTCTGTCGATACCCCACCGAGACCCCATTGGGGCCAATACGCCCGCGTTTCTTCCTTTTCCCCACCCCACCCCCCAAGTTCGGGTGAAGGCCCAGGGCTCGCAGCCAACGTCGGGGCGGCAGGCCCTGCCATAGCAGATCTGCGCAGCTGGGGCTCTAGGGGGTATCCCCACGCGCCCTGTAGCGGCGCATTAAGCGCGGCGGGTGTGGTGGTTACGCGCAGCGTGACCGCTACACTTGCCAGCGCCCTAGCGCCCGCTCCTTTCGCTTTCTTCCCTTCCTTTCTCGCCACGTTCGCCGGCTTTCCCCGTCAAGCTCTAAATCGGGGCATCCCTTTAGGGTTCCGATTTAGTGCTTTACGGCACCTCGACCCCAAAAAACTTGATTAGGGTGATGGTTCACGTAGTGGGCCATCGCCCTGATAGACGGTTTTTCGCCCTTTGACGTTGGAGTCCACGTTCTTTAATAGTGGACTCTTGTTCCAAACTGGAACAACACTCAACCCTATCTCGGTCTATTCTTTTGATTTATAAGGGATTTTGGGGATTTCGGCCTATTGGTTAAAAAATGAGCTGATTTAACAAAAATTTAACGCGAATTAATTCTGTGGAATGTGTGTCAGTTAGGGTGTGGAAAGTCCCCAGGCTCCCCAGCAGGCAGAAGTATGCAAAGCATGCATCTCAATTAGTCAGCAACCAGGTGTGGAAAGTCCCCAGGCTCCCCAGCAGGCAGAAGTATGCAAAGCATGCATCTCAATTAGTCAGCAACCATAGTCCCGCCCCTAACTCCGCCCATCCCGCCCCTAACTCCGCCCAGTTCCGCCCATTCTCCGCCCCATGGCTGACTAATTTTTTTTATTTATGCAGAGGCCGAGGCCGCCTCTGCCTCTGAGCTATTCCAGAAGTAGTGAGGAGGCTTTTTTGGAGGCCTAGGCTTTTGCAAAAAGCTCCCGGGAGCTTGTATATCCATTTTCGGATCTGATCAAGAGACAGGATGAGGATCGTTTCGCATGATTGAACAAGATGGATTGCACGCAGGTTCTCCGGCCGCTTGGGTGGAGAGGCTATTCGGCTATGACTGGGCACAACAGACAATCGGCTGCTCTGATGCCGCCGTGTTCCGGCTGTCAGCGCAGGGGCGCCCGGTTCTTTTTGTCAAGACCGACCTGTCCGGTGCCCTGAATGAACTGCAGGACGAGGCAGCGCGGCTATCGTGGCTGGCCACGACGGGCGTTCCTTGCGCAGCTGTGCTCGACGTTGTCACTGAAGCGGGAAGGGACTGGCTGCTATTGGGCGAAGTGCCGGGGCAGGATCTCCTGTCATCTCACCTTGCTCCTGCCGAGAAAGTATCCATCATGGCTGATGCAATGCGGCGGCTGCATACGCTTGATCCGGCTACCTGCCCATTCGACCACCAAGCGAAACATCGCATCGAGCGAGCACGTACTCGGATGGAAGCCGGTCTTGTCGATCAGGATGATCTGGACGAAGAGCATCAGGGGCTCGCGCCAGCCGAACTGTTCGCCAGGCTCAAGGCGCGCATGCCCGACGGCGAGGATCTCGTCGTGACCCATGGCGATGCCTGCTTGCCGAATATCATGGTGGAAAATGGCCGCTTTTCTGGATTCATCGACTGTGGCCGGCTGGGTGTGGCGGACCGCTATCAGGACATAGCGTTGGCTACCCGTGATATTGCTGAAGAGCTTGGCGGCGAATGGGCTGACCGCTTCCTCGTGCTTTACGGTATCGCCGCTCCCGATTCGCAGCGCATCGCCTTCTATCGCCTTCTTGACGAGTTCTTCTGAGCGGGACTCTGGGGTTCGCGAAATGACCGACCAAGCGACGCCCAACCTGCCATCACGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGGTTGGGCTTCGGAATCGTTTTCCGGGACGCCGGCTGGATGATCCTCCAGCGCGGGGATCTCATGCTGGAGTTCTTCGCCCACCCCAACTTGTTTATTGCAGCTTATAATGGTTACAAATAAAGCAATAGCATCACAAATTTCACAAATAAAGCATTTTTTTCACTGCATTCTAGTTGTGGTTTGTCCAAACTCATCAATGTATCTTATCATGTCTGTATACCGTCGACCTCTAGCTAGAGCTTGGCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCAATGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGACCCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACATTTCCCCGAAAAGTGCCACCTGACGTCGACGGATCGGGAGATCTCCCGATCCCCTATGGTCGACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGTATCTGCTCCCTGCTTGTGTGTTGGAGGTCGCTGAGTAGTGCGCGAGCAAAATTTAAGCTACAACAAGGCAAGGCTTGACCGACAATTGCATGAAGAATCTGCTTAGG
Gene Length
11556
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