PLV3-CMV-Ssh1 (mouse) -3xFLAG-CopGFP-Puro
Product Specifications
Applications
Protein Expression
Storage Temperature
37°C
Reference Sequence ID
NM_198109.5
Aliases
Gm1394; Gm1395; SSH-1; SSH-1L
Plasmid Hos
Mammalian cell, Lentivirus
Inserted Fragment Type
ORF
Inserted Fragment Species
Mouse
Resistance
Amp
Selection antibiotic
Puro
Fluorescent labeling
Green
Competent cells
Stbl3
Nucleic Acid Sequence
AcgcgtgtagtcttatgcaatactcttgtagtcttgcaacatggtaacgatgagttagcaacatgccttacaaggagagaaaaagcaccgtgcatgccgattggtggaagtaaggtggtacgatcgtgccttattaggaaggcaacagacgggtctgacatggattggacgaaccactgaattgccgcattgcagagatattgtatttaagtgcctagctcgatacaataaacgggtctctctggttagaccagatctgagcctgggagctctctggctaactagggaacccactgcttaagcctcaataaagcttgccttgagtgcttcaagtagtgtgtgcccgtctgttgtgtgactctggtaactagagatccctcagacccttttagtcagtgtggaaaatctctagcagtggcgcccgaacagggacctgaaagcgaaagggaaaccagagctctctcgacgcaggactcggcttgctgaagcgcgcacggcaagaggcgaggggcggcgactggtgagtacgccaaaaattttgactagcggaggctagaaggagagagatgggtgcgagagcgtcagtattaagcgggggagaattagatcgcgatgggaaaaaattcggttaaggccagggggaaagaaaaaatataaattaaaacatatagtatgggcaagcagggagctagaacgattcgcagttaatcctggcctgttagaaacatcagaaggctgtagacaaatactgggacagctacaaccatcccttcagacaggatcagaagaacttagatcattatataatacagtagcaaccctctattgtgtgcatcaaaggatagagataaaagacaccaaggaagctttagacaagatagaggaagagcaaaacaaaagtaagaccaccgcacagcaagcggccactgatcttcagacctggaggaggagatatgagggacaattggagaagtgaattatataaatataaagtagtaaaaattgaaccattaggagtagcacccaccaaggcaaagagaagagtggtgcagagagaaaaaagagcagtgggaataggagctttgttccttgggttcttgggagcagcaggaagcactatgggcgcagcctcaatgacgctgacggtacaggccagacaattattgtctggtatagtgcagcagcagaacaatttgctgagggctattgaggcgcaacagcatctgttgcaactcacagtctggggcatcaagcagctccaggcaagaatcctggctgtggaaagatacctaaaggatcaacagctcctggggatttggggttgctctggaaaactcatttgcaccactgctgtgccttggaatgctagttggagtaataaatctctggaacagattggaatcacacgacctggatggagtgggacagagaaattaacaattacacaagcttaatacactccttaattgaagaatcgcaaaaccagcaagaaaagaatgaacaagaattattggaattagataaatgggcaagtttgtggaattggtttaacataacaaattggctgtggtatataaaattattcataatgatagtaggaggcttggtaggtttaagaatagtttttgctgtactttctatagtgaatagagttaggcagggatattcaccattatcgtttcagacccacctcccaaccccgaggggacccgacaggcccgaaggaatagaagaagaaggtggagagagagacagagacagatccattcgattagtgaacggatctcgacggtATCGGTtaacttttaaaagaaaaggggggattggggggtacagtgcaggggaaagaatagtagacataatagcaacagacatacaaactaaagaattacaaaaacaaattacaaaaattcaaaattttatcgatactagtattatgcccagtacatgaccttatgggactttcctacttggcagtacatctacgtattagtcatcgctattaccatggtgatgcggttttggcagtacatcaatgggcgtggatagcggtttgactcacggggatttccaagtctccaccccattgacgtcaatgggagtttgttttggcaccaaaatcaacgggactttccaaaatgtcgtaacaactccgccccattgacgcaaatgggcggtaggcgtgtacggtgggaggtTtatataagcagagctcgtttagtgaaccgtcagatcgcctggagacgccatccacgctgttttgacctccatagaagattctagagctagcCTGATACGAACTCGgaattcgccaccatggccttggtgacccttcagcgctctcccacgcccagcgccgcgtcttcctccgcaagcaacagcgagttggaggctggcagcgatgaagaacggaaattgaacctcagcttgagtgagagctttttcatggtgaaaggagctgccctcttcctacagcagggaaacagcccacagggccagcggagtcttcagcaccctcacaagcatgcaggtgatctgcctcagcacctgcaagtgatgatcaacctcctgcgttgtgaagacagaatcaagctggccgtgcgcctagagagtgtctggaccgaccgtgtccgctacatggtcgtggtatacaccagcgggcgccaggacaccgaggagaatattctgctgggagttgacttttccagtaaggagagcaaaagctgcacgatcggaatggttcttcgactctggagcgacaccaagattcacctcgatggggacggcgggttcagtgtgagcacagcgggcaggatgcacatattcaagccagtgtctgtccaggccatgtggtctgccctgcaagtgcttcacaaggcctgcgaagtggcccggaggcataactacttcccaggaggagtggcgctcatctgggccacctactatgagagctgcatcagctcggagcagagctgcatcaatgagtggaatgccatgcaggacctggagtccacgcggcccgactcccccgcgctgtttgtggacaagccaaccgagggcgaaagaactgagcgcctcattaaagccaaactccggagcatcatgatgagccaggaccttgaaaatgtgacttctaaggaaatccgaaatgagctggagaagcaaatgaactgcaacctgaaggagttcaaggaattcatcgataacgagatgctgctcatcctgggccagatggacaagccctccctcatctttgaccatctttatcttggctccgagtggaatgcatccaatctggaggagctgcagggctcaggagttgactacattttaaatgtcactagagaaatagacaattttttccccggcttgtttgcgtaccataacatccgcgtgtacgatgaggagaccacagaccttcttgcccactggaatgaagcttatcatttcataaacaaagcgaaaaggaatcattccaagtgcctggtccattgcaagatgggcgtcagccgatctgcgtccacagtcattgcctacgccatgaaggagttcggctggcccctggagaaagcgtataactacgtgaagcagaaacgtagtatcactcggcccaacgcaggctttatgaggcagctgtctgagtacgaaggcatcctggatgcaagcaaacagcggcataacaaactatggcgccagcagcccacagatgacaccatcgcagagcccagtgagttcttgccagagaccttggacggagccctggacgctcagctgccctgtttggatgataccacccaccctgggctcccgagaagtctggccccaggaggacccgctctcccctgttgttttcgaagactctcggaccccctcctccttccccaccatgatgaaacgggtggcctggtccacttagaggatctcgagaaggatgctctgttagaggaggaggagtctcagccagtggaggtgcacaagctggttcagcatccccaggaaggtgccaggctgtgtgaaaaggacgtaaagaggaaactggagtttgggaactccaaaccccgcagtgactccttgcctcaggtggaggagctggagaaggacggtagcccaagaacggggaggtggaggcgggcctccacccagctcgatagaagcttgctcgaccaggaaaacctaaataacaacaacagcaagaggagctgtcccgatgacttggagcgcgatgccatgtttggaattctcagcaaagtgaagcctccctacacatcctgtgccgactgcatgtaccccacagctggtgggactcccgaggcctacatggagcgacacgaagaccccagctcctctgctatctgcacccagccaaccttcctcccccatgtcacgtcttccccaatggcccacgcgagcagcaggtcccgagctccggagaggccggcctcgggtccagccaacacctctccattcctactaccagcaggctccaggaagccagatgtcagtggctctggagccggggctgccccggaaccaccagcaagccttctagagccttccagagagacctcaaaagcccttccaaaatctctccagttgaagaatcctcactgtgacaagaacgctgccaatatggaggtgtcggcgaaggaagaaccgtcgcctaagaaagatcccaagccggctaaggacctgaggcttctgttcagtaacgaggcggagaagcccaccaccaacagctacctgatgcagcatcaggagtccatcatccaactgcagaaggcaggcctggtccgaaagcacaccaaagagctggagaggttgaagagcctgccttcagactcaccagctgcctgcagggacagcgccacctgcaggctggaggccagcatcccggaggagggtagccaggagcccgcacacccagccctgtgcagccaagctgggtcggaagaacagcctgtagggggaaccttgcagaagagccccacgtctaccctcccccgtttagatcacaccagtaacttctcgaaggacttcctgaagaccgtgtgttacacccccacctcctcctccatcagctccaacctgacccggagctccagcagcgacagcatccacagcgtccgagggaagcctgggctggtgaagcagcgggcgcaggagatcgagacgcggctgcgcctggcaggcctcaccgtgtcgtccccgctgaaaaggtcccattcccttgccaagctgggaagtctcaacttctccacggaggacctgtccagcgaggctgacacatccaccatcgctgactcgcaggacgccaagtgtggtctctcctcttccttcttgcccgaaccccagtctgcgccaagggaccccgctgcaacctctaaatcatcagggaaatctgccccagaacacttgaaaagcccgtcgagggtaaacaaaagcggatccGACTACAAAGACCATGACGGTGATTATAAAGATCATGACATCGACTACAAGGATGACGATGACAAGtagcggccgcGaaggatctgcgatcgctccggtgcccgtcagtgggcagagcgcacatcgcccacagtccccgagaagttggggggaggggtcggcaattgaacgggtgcctagagaaggtggcgcggggtaaactgggaaagtgatgtcgtgtactggctccgcctttttcccgagggtgggggagaaccgtatataagtgcagtagtcgccgtgaacgttctttttcgcaacgggtttgccgccagaacacagctgaagcttcgaggggctcgcatctctccttcacgcgcccgccgccctacctgaggccgccatccacgccggttgagtcgcgttctgccgcctcccgcctgtggtgcctcctgaactgcgtccgccgtctaggtaagtttaaagctcaggtcgagaccgggcctttgtccggcgctcccttggagcctacctagactcagccggctctccacgctttgcctgaccctgcttgctcaactctacgtctttgtttcgttttctgttctgcgccgttacagatccaagctgtgaccggcgcctacgctagacgccaccatggagagcgacgagagcggcctgcccgccatggagatcgagtgccgcatcaccggcaccctgaacggcgtggagttcgagctggtgggcggcggagagggcacccccaagcagggccgcatgaccaacaagatgaagagcaccaaaggcgccctgaccttcagcccctacctgctgagccacgtgatgggctacggcttctaccacttcggcacctaccccagcggctacgagaaccccttcctgcacgccatcaacaacggcggctacaccaacacccgcatcgagaagtacgaggacggcggcgtgctgcacgtgagcttcagctaccgctacgaggccggccgcgtgatcggcgacttcaaggtggtgggcaccggcttccccgaggacagcgtgatcttcaccgacaagatcatccgcagcaacgccaccgtggagcacctgcaccccatgggcgataacgtgctggtgggcagcttcgcccgcaccttcagcctgcgcgacggcggctactacagcttcgtggtggacagccacatgcacttcaagagcgccatccaccccagcatcctgcagaacgggggccccatgttcgccttccgccgcgtggaggagctgcacagcaacaccgagctgggcatcgtggagtaccagcacgccttcaagacccccatcgccttcgccagatcccgcgctcagtcgtccaattctgccgtggacggcaccgccggacccggctccaccggatctcgcgagggcagaggaagtcttctaacatgcggtgacgtggaggagaatcccggccctatgaccgagtacaagcccacggtgcgcctcgccacccgcgacgacgtccccagggccgtacgcaccctcgccgccgcgttcgccgactaccccgccacgcgccacaccgtcgatccggaccgccacatcgagcgggtcaccgagctgcaagaactcttcctcacgcgcgtcgggctcgacatcggcaaggtgtgggtcgcggacgacggcgccgcggtggcggtctggaccacgccggagagcgtcgaagcgggggcggtgttcgccgagatcggcccgcgcatggccgagttgagcggttcccggctggccgcgcagcaacagatggaaggcctcctggcgccgcaccggcccaaggagcccgcgtggttcctggccaccgtcggcgtctcgcccgaccaccagggcaagggtctgggcagcgccgtcgtgctccccggagtggaggcggccgagcgcgccggggtgcccgccttcctggagacctccgcgccccgcaacctccccttctacgagcggctcggcttcaccgtcaccgccgacgtcgaggtgcccgaaggaccgcgcacctggtgcatgacccgcaagcccggtgcctgaaatcaacctctggattacaaaatttgtgaaagattgactggtattcttaactatgttgctccttttacgctatgtggatacgctgctttaatgcctttgtatcatgctattgcttcccgtatggctttcattttctcctccttgtataaatcctggttgctgtctctttatgaggagttgtggcccgttgtcaggcaacgtggcgtggtgtgcactgtgtttgctgacgcaacccccactggttggggcattgccaccacctgtcagctcctttccgggactttcgctttccccctccctattgccacggcggaactcatcgccgcctgccttgcccgctgctggacaggggctcggctgttgggcactgacaattccgtggtgttgtcggggaaATCATCGTCCTTTCCTtggctgctcgcctgtgttgccacctggattctgcgcgggacgtccttctgctacgtcccttcggccctcaatccagcggaccttccttcccgcggcctgctgccggctctgcggcctcttccgcgtctTcgccttcgccctcagacgagtcggatctccctttggGccgcctccccgcctggtacctttaagaccaatgacttacaaggcagctgtagatcttagccactttttaaaagaaaaggggggactggaagggctaattcactcccaacgaaGataagatctgctttttgcttgtactgggtctctctggttagaccagatctgagcctgggagctctctggctaactagggaacccactgcttaagcctcaataaagcttgccttgagtgcttcaagtagtgtgtgcccgtctgttgtgtgactctggtaactagagatccctcagacccttttagtcagtgtggaaaatctctagcagtagtagttcatgtcatcttattattcagtatttataacttgcaaagaaatgaatatcagagagtgagaggaacttgtttattgcagcttataatggttacaaataaagcaatagcatcacaaatttcacaaataaagcatttttttcactgcattctagttgtggtttgtccaaactcatcaatgtatcttatcatgtctggctctagctaTCCCGCCCCTAACTCCGCCCAtcccgcccctaactccgcccagttccgcccattctccgccccatggctgactaattttttttatttatgcagaggccgaggccgcctcggcctctgagctattccagaagtagtgaggaggcttttttggaggcctagacttttgcagagaccaaattcgtaatcatgtcatagctgtttcctgtgtgaaattgttatccgctcacaattccacacaacatacgagccggaagcataaagtgtaaagcctggggtgcctaatgagtgagctaactcacattaattgcgttgcgctcactgcccgctttccagtcgggaaacctgtcgtgccagctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgctcttccgcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccataggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaaggacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagttaccaatgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagtgctgcaatgataccgcgagacccacgctcaccggctccagatttatcagcaataaaccagccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctattaattgttgccgggaagctagagtaagtagttcgccagttaatagtttgcgcaacgttgttgccattgctacaggcatcgtggtgtcacgctcgtcgtttggtatggcttcattcagctccggttcccaacgatcaaggcgagttacatgatcccccatgttgtgcaaaaaagcggttagctccttcggtcctccgatcgttgtcagaagtaagttggccgcagtgttatcactcatggttatggcagcactgcataattctcttactgtcatgccatccgtaagatgcttttctgtgactggtgagtactcaaccaagtcattctgagaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaatacgggataataccgcgccacatagcagaactttaaaagtgctcatcattggaaaacgttcttcggggcgaaaactctcaaggatcttaccgctgttgagatccagttcgatgtaacccactcgtgcacccaactgatcttcagcatcttttactttcaccagcgtttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcgacacggaaatgttgaatactcatactcttcctttttcaatattattgaagcatttatcagggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggggttccgcgcacatttccccgaaaagtgccacctgacgtctaagaaaccattattatcatgacattaacctataaaaataggcgtatcacgaggccctttcgtctcgcgcgtttcggtgatgacggtgaaaacctctgacacatgcagctcccggagacggtcacagcttgtctgtaagcggatgccgggagcagacaagcccgtcagggcgcgtcagcgggtgttggcgggtgtcggggctggcttaactatgcggcatcagagcagattgtactgagagtgcaccatatgcggtgtgaaataccgcacagatgcgtaaggagaaaataccgcatcaggcgccattcgccattcaggctgcgcaactgttgggaagggcgatcggtgcgggcctcttcgctattacgccagctggcgaaagggggatgtgctgcaaggcgattaagttgggtaacgccagggttttcccagtcacgacgttgtaaaacgacggccagtgccaagctg
Gene Length
11416
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LY401382 100 µg
B Raf (BRAF) (NM_004333) Human Over-expression Lysate
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